Systematic Study of Functionalizable, Non-Biofouling Agarose Films with Protein and Cellular Patterns on Glass Slides

Gyeongyeop Han, Daehwa Hong, Bong Soo Lee, Eun Rae Ha, Ji Hun Park, Insung S. Choi, Sung Min Kang, Jungkyu K. Lee

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Herein we demonstrate a systematic investigation of chemically functionalizable, non-biofouling agarose films over large-area glass surfaces. Agarose films, prepared with various concentrations of aqueous agarose, were activated by using periodate oxidation to generate aldehyde groups at the termini of the agarose chains. The non-biofouling efficacy and binding capabilities of the activated films were evaluated by using protein and cellular patterning, performed by using a microarrayer, microcontact printing, and micromolding in capillaries. Characterization by using a fluorescence slide scanner and a scanning-probe microscope revealed that the pore sizes of the agarose films played an important role in achieving desirable film performance; the 0.2 wt % agarose film exhibited the optimum efficacy in this work.

Original languageEnglish
Pages (from-to)846-852
Number of pages7
JournalChemistry - An Asian Journal
Volume12
Issue number8
DOIs
StatePublished - 18 Apr 2017

Bibliographical note

Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • bioorganic chemistry
  • coating techniques
  • protein and cellular pattern
  • surface chemistry
  • thin films

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