Synthesis of sub 3 nm-sized uniform magnetite nanoparticles using reverse micelle method for biomedical application

Euiyoung Jung, Sung Won Kim, Ahyoung Cho, Yu Jin Kim, Gun Jae Jeong, Jinheung Kim, Suk Ho Bhang, Taekyung Yu

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


We report a synthetic method for small and uniform Fe3O4 (magnetite) nanoparticles under mild conditions. Spherical sub-3 nm-sized magnetite nanoparticles were prepared via reverse micelles composed of oleylamine, F127, xylene, and water for the reaction of iron(III) stearate with hydrazine at a reaction temperature of 90 °C in air atmosphere. These synthesized magnetite nanoparticles exhibited good size uniformity. By controlling experimental conditions, we could easily control both size and size uniformity of these magnetite nanoparticles. We further investigated whether Fe3O4 could be used in biomedical applications. Cytotoxicity of Fe3O4 was evaluated with human adipose-derived stem cells (hADSCs). Our results showed that the number of hADSCs did not significantly decrease when these cells were treated with Fe3O4 nanoparticles at a concentration of up to 9 μg/mL. Apoptotic activity and cell proliferation of hADSCs treated with Fe3O4 nanoparticles were similar to those of hADSCs without any treatment. This novel method could be used for synthesizing uniform and biocompatible Fe3O4 nanoparticles with further biomedical applications.

Original languageEnglish
Article number3850
Issue number23
StatePublished - 1 Dec 2019

Bibliographical note

Funding Information:
This research was supported by grants (NRF-2014R1A5A1009799, NRF-2019R1H1A2079332, NRF-2016M3D1A1021140, NRF-2017R1A5A1070259, 2016M3A9B4919711, and 2019R1C1C1007384) of the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning. This work was also supported by Next Generation Carbon Upcycling Project (2017M1A2A2046740) through the NRF funded by the Ministry of Science and ICT, Korea.

Publisher Copyright:
© 2019 by the authors.


  • Iron oxide
  • Magnetite
  • Nanoparticles
  • Reverse micelle
  • Sub-3 nm


Dive into the research topics of 'Synthesis of sub 3 nm-sized uniform magnetite nanoparticles using reverse micelle method for biomedical application'. Together they form a unique fingerprint.

Cite this