Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli

Si Jae Park, Eun Young Kim, Won Noh, Young Hoon Oh, Hye Young Kim, Bong Keun Song, Kwang Myung Cho, Soon Ho Hong, Seung Hwan Lee, Jonggeon Jegal

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD600 of 2-10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD600 of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al 2O3 as catalyst in toluene with the yield of 96 %.

Original languageEnglish
Pages (from-to)885-892
Number of pages8
JournalBioprocess and Biosystems Engineering
Volume36
Issue number7
DOIs
StatePublished - Jul 2013

Keywords

  • 2-Pyrrolodine
  • Bioconversion
  • Gamma-aminobutyrate (GABA)
  • Glutamate decarboxylase
  • MSG
  • Nylon 4
  • Recombinant E. coli

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