Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli

Si Jae Park; Eun Young Kim; Won Noh; Young Hoon Oh; Hye Young Kim; Bong Keun Song; Kwang Myung Cho; Soon Ho Hong; Seung Hwan Lee; Jonggeon Jegal

doi:10.1007/s00449-012-0821-2

Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli

Si Jae Park, Eun Young Kim, Won Noh, Young Hoon Oh, Hye Young Kim, Bong Keun Song, Kwang Myung Cho, Soon Ho Hong, Seung Hwan Lee, Jonggeon Jegal

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD₆₀₀ of 2-10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD₆₀₀ of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al ₂O₃ as catalyst in toluene with the yield of 96 %.

Original language	English
Pages (from-to)	885-892
Number of pages	8
Journal	Bioprocess and Biosystems Engineering
Volume	36
Issue number	7
DOIs	https://doi.org/10.1007/s00449-012-0821-2
State	Published - Jul 2013

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the R&D Program of MKE/KEIT (10033199 and 10033386) and a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ008057), Rural Development Administration, Republic of Korea. Further support from Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the MEST (2012-0006693) is appreciated.

Keywords

2-Pyrrolodine
Bioconversion
Gamma-aminobutyrate (GABA)
Glutamate decarboxylase
MSG
Nylon 4
Recombinant E. coli

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10.1007/s00449-012-0821-2

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@article{f3e93d0d87c5435fbd50e97ff373f37a,

title = "Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli",

abstract = "In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD600 of 2-10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD600 of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al 2O3 as catalyst in toluene with the yield of 96 %.",

keywords = "2-Pyrrolodine, Bioconversion, Gamma-aminobutyrate (GABA), Glutamate decarboxylase, MSG, Nylon 4, Recombinant E. coli",

author = "Park, {Si Jae} and Kim, {Eun Young} and Won Noh and Oh, {Young Hoon} and Kim, {Hye Young} and Song, {Bong Keun} and Cho, {Kwang Myung} and Hong, {Soon Ho} and Lee, {Seung Hwan} and Jonggeon Jegal",

note = "Funding Information: Acknowledgments This work was supported by the R&D Program of MKE/KEIT (10033199 and 10033386) and a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ008057), Rural Development Administration, Republic of Korea. Further support from Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the MEST (2012-0006693) is appreciated.",

year = "2013",

month = jul,

doi = "10.1007/s00449-012-0821-2",

language = "English",

volume = "36",

pages = "885--892",

journal = "Bioprocess and Biosystems Engineering",

issn = "1615-7591",

publisher = "Springer Verlag",

number = "7",

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T1 - Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli

AU - Park, Si Jae

AU - Kim, Eun Young

AU - Noh, Won

AU - Oh, Young Hoon

AU - Kim, Hye Young

AU - Song, Bong Keun

AU - Cho, Kwang Myung

AU - Hong, Soon Ho

AU - Lee, Seung Hwan

AU - Jegal, Jonggeon

N1 - Funding Information: Acknowledgments This work was supported by the R&D Program of MKE/KEIT (10033199 and 10033386) and a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ008057), Rural Development Administration, Republic of Korea. Further support from Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the MEST (2012-0006693) is appreciated.

PY - 2013/7

Y1 - 2013/7

N2 - In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD600 of 2-10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD600 of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al 2O3 as catalyst in toluene with the yield of 96 %.

AB - In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD600 of 2-10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD600 of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al 2O3 as catalyst in toluene with the yield of 96 %.

KW - 2-Pyrrolodine

KW - Bioconversion

KW - Gamma-aminobutyrate (GABA)

KW - Glutamate decarboxylase

KW - MSG

KW - Nylon 4

KW - Recombinant E. coli

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U2 - 10.1007/s00449-012-0821-2

DO - 10.1007/s00449-012-0821-2

M3 - Article

C2 - 23010721

AN - SCOPUS:84879554383

SN - 1615-7591

VL - 36

SP - 885

EP - 892

JO - Bioprocess and Biosystems Engineering

JF - Bioprocess and Biosystems Engineering

IS - 7

ER -

Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli

Abstract

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Keywords

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