Synthesis of α-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase

Heejin Kim, Nakyung Choi, Se Wook Oh, Yangha Kim, Byung Hee Kim, In Hwan Kim

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


An α-linolenic acid (ALA)-rich triacylglycerol (TAG) was synthesized from an ALA-rich fatty acid (FA) from perilla oil and glycerol, using a newly prepared immobilized lipase under vacuum. The ALA-rich FA (purity >90 wt%) used as the substrate was prepared by urea complexation from perilla oil FAs. Liquid Lipozyme TL 100 L lipase from Thermomyces lanuginosus was used for immobilization. Nine different hydrophilic and hydrophobic carriers for immobilization were tested, and Duolite A568, which is a hydrophilic resin, was selected as the best carrier. This immobilized lipase was used to synthesize TAG by direct esterification under vacuum. The parameters investigated were temperature, enzyme loading, and vacuum level. The optimum reaction conditions were a temperature of 60 °C, an enzyme loading of 15% (based on the total weight of the substrate), and a vacuum of 0.7 kPa, respectively. The maximum conversion to TAG of ca. 88 wt% was obtained in 12 h under the optimum conditions.

Original languageEnglish
Pages (from-to)654-658
Number of pages5
JournalFood Chemistry
StatePublished - 15 Dec 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Ltd


  • Duolite A568
  • Immobilization
  • Lipase
  • Thermomyces lanuginosus
  • Triacylglycerol
  • α-Linolenic acid


Dive into the research topics of 'Synthesis of α-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase'. Together they form a unique fingerprint.

Cite this