Synthesis and characterization of novel thermo-responsive F68 block copolymers with cell-adhesive RGD peptide

Myoung Hwa Cha; Jiyeon Choi; Bo Gyu Choi; Kwideok Park; Ik Hwan Kim; Byeongmoon Jeong; Dong Keun Han

doi:10.1016/j.jcis.2011.04.041

Synthesis and characterization of novel thermo-responsive F68 block copolymers with cell-adhesive RGD peptide

Myoung Hwa Cha, Jiyeon Choi, Bo Gyu Choi, Kwideok Park, Ik Hwan Kim, Byeongmoon Jeong, Dong Keun Han

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Thermosensitive poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer, Pluronic F68, containing a hydrophobic unit, oligo-(lactic acid)(oligo-LA) or oligo-caprolactone (oligo-CL), 2-META and RGD as side groups was successfully synthesized and characterized by ¹H NMR, FTIR, and elemental analysis. Their aqueous solution displayed special gel-sol-gel phase transition behavior with increasing temperature from 10 to 70°C, when the polymer concentration was above critical micelle concentration (CMC). The gel-sol phase diagram was investigated using tube inversion method, rheological measurement, and dynamic light scattering. Based on these results, the gelation properties of modified F68 were affected by several factors such as the composition of the substituents, chain length of oligo l-LA or oligo -CL, and the concentration of the polymer solutions. The unique phase transition behavior with temperature was observed by modified F68 triblock copolymer, composed of the PPO blocks core and the PEO blocks shell in aqueous solution. This phenomenon was elucidated using ¹H NMR data; the alteration of hydrophobic interaction and chain mobility led to the formation of transparent gel, coexistence of gel-sol, and opaque gel. These hydrogels may be useful in drug delivery and tissue engineering.

Original language	English
Pages (from-to)	78-85
Number of pages	8
Journal	Journal of Colloid and Interface Science
Volume	360
Issue number	1
DOIs	https://doi.org/10.1016/j.jcis.2011.04.041
State	Published - 1 Aug 2011

Bibliographical note

Funding Information:
This research was supported by Pioneer Research Center Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2010-0002170), and a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea (K00060-282).

Keywords

Biodegradable polymer
Gel-sol transition
Pluronic F68
RGD peptide
Thermosensitive hydrogel

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10.1016/j.jcis.2011.04.041

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title = "Synthesis and characterization of novel thermo-responsive F68 block copolymers with cell-adhesive RGD peptide",

abstract = "Thermosensitive poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer, Pluronic F68, containing a hydrophobic unit, oligo-(lactic acid)(oligo-LA) or oligo-caprolactone (oligo-CL), 2-META and RGD as side groups was successfully synthesized and characterized by 1H NMR, FTIR, and elemental analysis. Their aqueous solution displayed special gel-sol-gel phase transition behavior with increasing temperature from 10 to 70°C, when the polymer concentration was above critical micelle concentration (CMC). The gel-sol phase diagram was investigated using tube inversion method, rheological measurement, and dynamic light scattering. Based on these results, the gelation properties of modified F68 were affected by several factors such as the composition of the substituents, chain length of oligo l-LA or oligo -CL, and the concentration of the polymer solutions. The unique phase transition behavior with temperature was observed by modified F68 triblock copolymer, composed of the PPO blocks core and the PEO blocks shell in aqueous solution. This phenomenon was elucidated using 1H NMR data; the alteration of hydrophobic interaction and chain mobility led to the formation of transparent gel, coexistence of gel-sol, and opaque gel. These hydrogels may be useful in drug delivery and tissue engineering.",

keywords = "Biodegradable polymer, Gel-sol transition, Pluronic F68, RGD peptide, Thermosensitive hydrogel",

author = "Cha, {Myoung Hwa} and Jiyeon Choi and Choi, {Bo Gyu} and Kwideok Park and Kim, {Ik Hwan} and Byeongmoon Jeong and Han, {Dong Keun}",

note = "Funding Information: This research was supported by Pioneer Research Center Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2010-0002170), and a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea (K00060-282).",

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AU - Cha, Myoung Hwa

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AU - Choi, Bo Gyu

AU - Park, Kwideok

AU - Kim, Ik Hwan

AU - Jeong, Byeongmoon

AU - Han, Dong Keun

N1 - Funding Information: This research was supported by Pioneer Research Center Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2010-0002170), and a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea (K00060-282).

PY - 2011/8/1

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N2 - Thermosensitive poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer, Pluronic F68, containing a hydrophobic unit, oligo-(lactic acid)(oligo-LA) or oligo-caprolactone (oligo-CL), 2-META and RGD as side groups was successfully synthesized and characterized by 1H NMR, FTIR, and elemental analysis. Their aqueous solution displayed special gel-sol-gel phase transition behavior with increasing temperature from 10 to 70°C, when the polymer concentration was above critical micelle concentration (CMC). The gel-sol phase diagram was investigated using tube inversion method, rheological measurement, and dynamic light scattering. Based on these results, the gelation properties of modified F68 were affected by several factors such as the composition of the substituents, chain length of oligo l-LA or oligo -CL, and the concentration of the polymer solutions. The unique phase transition behavior with temperature was observed by modified F68 triblock copolymer, composed of the PPO blocks core and the PEO blocks shell in aqueous solution. This phenomenon was elucidated using 1H NMR data; the alteration of hydrophobic interaction and chain mobility led to the formation of transparent gel, coexistence of gel-sol, and opaque gel. These hydrogels may be useful in drug delivery and tissue engineering.

AB - Thermosensitive poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer, Pluronic F68, containing a hydrophobic unit, oligo-(lactic acid)(oligo-LA) or oligo-caprolactone (oligo-CL), 2-META and RGD as side groups was successfully synthesized and characterized by 1H NMR, FTIR, and elemental analysis. Their aqueous solution displayed special gel-sol-gel phase transition behavior with increasing temperature from 10 to 70°C, when the polymer concentration was above critical micelle concentration (CMC). The gel-sol phase diagram was investigated using tube inversion method, rheological measurement, and dynamic light scattering. Based on these results, the gelation properties of modified F68 were affected by several factors such as the composition of the substituents, chain length of oligo l-LA or oligo -CL, and the concentration of the polymer solutions. The unique phase transition behavior with temperature was observed by modified F68 triblock copolymer, composed of the PPO blocks core and the PEO blocks shell in aqueous solution. This phenomenon was elucidated using 1H NMR data; the alteration of hydrophobic interaction and chain mobility led to the formation of transparent gel, coexistence of gel-sol, and opaque gel. These hydrogels may be useful in drug delivery and tissue engineering.

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KW - RGD peptide

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Synthesis and characterization of novel thermo-responsive F68 block copolymers with cell-adhesive RGD peptide

Abstract

Bibliographical note

Keywords

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