Abstract
A series of 1-substituted-3(5)-(6-methylpyridin-2-yl)-4-(quinoxalin-6-yl) pyrazoles 14a-d, 15a-d, 17a, 17b, 18a-d, 19a, and 19b has been synthesized and evaluated for their ALK5 inhibitory activity in an enzyme assay and in a cell-based luciferase reporter assay. The 2-[3-(6-methylpyridin-2-yl)-4- (quinoxalin-6-yl)-1H-pyrazol-1-yl]-N-phenylethanethioamide (18a) inhibited ALK5 phosphorylation with an IC 50 value of 0.013 μM and showed 80% inhibition at 0.1 μM in a luciferase reporter assay using HaCaT cells permanently transfected with p3TP-luc reporter construct.
Original language | English |
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Pages (from-to) | 3917-3925 |
Number of pages | 9 |
Journal | European Journal of Medicinal Chemistry |
Volume | 46 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2011 |
Bibliographical note
Funding Information:This work was supported by RP-Grant 2009 of Ewha Womans University and a grant from Ministry of Education, Science and Technology, Korea ( 20100029596 ).
Keywords
- ALK5 inhibitor
- Cancer
- Cell-based luciferase reporter assay
- Docking
- Fibrosis
- Kinase assay
- TGF-β