Syndecan-4 regulates platelet-derived growth factor-mediated MAP kinase activation by altering intracellular reactive oxygen species

Jungyean Kim, Jung hyun Lee, Hey Sun Park, Jisun Hwang, Inn Oc Han, Yun Soo Bae, Eok Soo Oh

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The cell adhesion receptor, syndecan-4, regulates cellular interactions with both the extracellular matrix and soluble ligands. Accumulating evidence also suggests that cell adhesion is involved in generating reactive oxygen species (ROS). Here, we investigated the role of syndecan-4 in regulating growth factor-induced ROS generation. Rat embryo fibroblasts (REFs) overexpressing syndecan-4 exhibited increased ROS levels compared to control cells. Expression of the non-phagocytic NADH oxidase component Nox1 was increased in syndecan-4-overexpressing REFs and syndecan-4-mediated ROS generation was diminished when levels of Nox1 were knocked-down with small inhibitory RNAs. In addition, syndecan-4 enhanced platelet-derived growth factor (PDGF)-induced MAP kinase activity in parallel with ROS generation. Collectively, these data suggest that syndecan-4 regulates PDGF-induced MAP kinase activation by altering ROS generation.

Original languageEnglish
Pages (from-to)2725-2730
Number of pages6
JournalFEBS Letters
Volume582
Issue number18
DOIs
StatePublished - 6 Aug 2008

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-2002-CP0327 to ESO), and in part by a Grant No. R15-2006-020 from the NCRC program of the MOST and the KOSEF through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University.

Keywords

  • MAP kinase
  • Platelet-derived growth factor
  • Reactive oxygen species
  • Syndecan-4

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