TY - JOUR
T1 - Syndecan-2 regulates the migratory potential of melanoma cells
AU - Lee, Jung Hyun
AU - Park, Haein
AU - Chung, Heesung
AU - Choi, Sojoong
AU - Kim, Younghwa
AU - Yoo, Hyun
AU - Kim, Tae Yoon
AU - Hann, Hoo Jae
AU - Seong, Ikjoo
AU - Kim, Jaesang
AU - Kang, Kathleen G.
AU - Han, Inn Oc
AU - Oh, Eok Soo
PY - 2009/10/2
Y1 - 2009/10/2
N2 - Syndecan-2, a transmembrane heparan sulfate proteoglycan, is a critical mediator in the tumorigenesis of colon carcinoma cells. We explored the function of syndecan-2 in melanoma, one of the most invasive types of cancers, and found that the expression of this protein was elevated in tissue samples from both nevus and malignant human melanomas but not in melanocytes of the normal human skin tissues. Similarly, elevated syndecan-2 expression was observed in various melanoma cell lines. Overexpression of syndecan-2 enhanced migration and invasion of melanoma cells, whereas the opposite was observed when syndecan-2 levels were knocked down using small inhibitory RNAs. Syndecan-2 expression was enhanced by fibroblast growth factor-2, which is known to stimulate melanoma cell migration; however, α-melanocyte-stimulating hormone decreased syndecan-2 expression and melanoma cell migration and invasion in a melanin synthesis-independent manner. Furthermore, syndecan-2 overexpression rescued the migration defects induced by α-melanocyte-stimulating hormone treatment. Together, these data strongly suggest that syndecan-2 plays a crucial role in the migratory potential of melanoma cells.
AB - Syndecan-2, a transmembrane heparan sulfate proteoglycan, is a critical mediator in the tumorigenesis of colon carcinoma cells. We explored the function of syndecan-2 in melanoma, one of the most invasive types of cancers, and found that the expression of this protein was elevated in tissue samples from both nevus and malignant human melanomas but not in melanocytes of the normal human skin tissues. Similarly, elevated syndecan-2 expression was observed in various melanoma cell lines. Overexpression of syndecan-2 enhanced migration and invasion of melanoma cells, whereas the opposite was observed when syndecan-2 levels were knocked down using small inhibitory RNAs. Syndecan-2 expression was enhanced by fibroblast growth factor-2, which is known to stimulate melanoma cell migration; however, α-melanocyte-stimulating hormone decreased syndecan-2 expression and melanoma cell migration and invasion in a melanin synthesis-independent manner. Furthermore, syndecan-2 overexpression rescued the migration defects induced by α-melanocyte-stimulating hormone treatment. Together, these data strongly suggest that syndecan-2 plays a crucial role in the migratory potential of melanoma cells.
UR - http://www.scopus.com/inward/record.url?scp=70350452116&partnerID=8YFLogxK
U2 - 10.1074/jbc.M109.034678
DO - 10.1074/jbc.M109.034678
M3 - Article
C2 - 19641225
AN - SCOPUS:70350452116
SN - 0021-9258
VL - 284
SP - 27167
EP - 27175
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 40
ER -