Syndecan-2 regulates cell migration in colon cancer cells through Tiam1-mediated Rac activation

Youngsil Choi; Hyunjung Kim; Heesung Chung; Ji Sun Hwang; Jin A. Shin; Inn Oc Han; Eok Soo Oh

doi:10.1016/j.bbrc.2009.11.165

Syndecan-2 regulates cell migration in colon cancer cells through Tiam1-mediated Rac activation

Youngsil Choi, Hyunjung Kim, Heesung Chung, Ji Sun Hwang, Jin A. Shin, Inn Oc Han, Eok Soo Oh

Life Sciences

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Expression of the cell surface adhesion receptor syndecan-2 is known to be involved in the regulation of cancer cell migration. However, the molecular mechanism of syndecan-2-mediated cell migration remains unknown. Here we report that Rac contributes to the regulation of syndecan-2-mediated cancer cell migration. Overexpression of syndecan-2 enhanced migration and invasion of human colon adenocarcinoma cells Caco-2 and HCT116 cells. In parallel with the increased cell migration/invasion, syndecan-2 overexpression enhanced Rac activity, while dominant negative Rac (RacN17) diminished syndecan-2-mediated increased cancer cell migration. In addition syndecan-2 expression increased membrane localization of Tiam1 and syndecan-2-mediated cell migration/invasion of Caco-2 cells was diminished when Tiam1 levels were knocked-down with small inhibitory RNAs. Furthermore, oligomerization-defective syndecan-2 mutants failed to increase membrane localization of Tiam1, activation of Rac and subsequent cell migration of both Caco-2 and HCT116 cells. Taken together, these results suggest that syndecan-2 regulates cell migration of colon carcinoma cells through Tiam1-dependent Rac activation in colon cancer cells.

Original language	English
Pages (from-to)	921-925
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.11.165
State	Published - 1 Jan 2010

Keywords

Cell migration
Colon cancer
Rac
Signal transduction
Syndecan-2

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2009.11.165

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title = "Syndecan-2 regulates cell migration in colon cancer cells through Tiam1-mediated Rac activation",

abstract = "Expression of the cell surface adhesion receptor syndecan-2 is known to be involved in the regulation of cancer cell migration. However, the molecular mechanism of syndecan-2-mediated cell migration remains unknown. Here we report that Rac contributes to the regulation of syndecan-2-mediated cancer cell migration. Overexpression of syndecan-2 enhanced migration and invasion of human colon adenocarcinoma cells Caco-2 and HCT116 cells. In parallel with the increased cell migration/invasion, syndecan-2 overexpression enhanced Rac activity, while dominant negative Rac (RacN17) diminished syndecan-2-mediated increased cancer cell migration. In addition syndecan-2 expression increased membrane localization of Tiam1 and syndecan-2-mediated cell migration/invasion of Caco-2 cells was diminished when Tiam1 levels were knocked-down with small inhibitory RNAs. Furthermore, oligomerization-defective syndecan-2 mutants failed to increase membrane localization of Tiam1, activation of Rac and subsequent cell migration of both Caco-2 and HCT116 cells. Taken together, these results suggest that syndecan-2 regulates cell migration of colon carcinoma cells through Tiam1-dependent Rac activation in colon cancer cells.",

keywords = "Cell migration, Colon cancer, Rac, Signal transduction, Syndecan-2",

author = "Youngsil Choi and Hyunjung Kim and Heesung Chung and Hwang, {Ji Sun} and Shin, {Jin A.} and Han, {Inn Oc} and Oh, {Eok Soo}",

note = "Funding Information: This study was supported by a grant of the Research at National R&D Program for Cancer Control, Ministry of Health & Welfare , Republic of Korea ( 0420070-1 to E.S.O.) and Grant No. R15-2006-020 from the NCRC program of the MOST and the KOSEF through the Center for Cell Signaling & Drug Discovery Ewha Womans University . ",

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AU - Choi, Youngsil

AU - Kim, Hyunjung

AU - Chung, Heesung

AU - Hwang, Ji Sun

AU - Shin, Jin A.

AU - Han, Inn Oc

AU - Oh, Eok Soo

N1 - Funding Information: This study was supported by a grant of the Research at National R&D Program for Cancer Control, Ministry of Health & Welfare , Republic of Korea ( 0420070-1 to E.S.O.) and Grant No. R15-2006-020 from the NCRC program of the MOST and the KOSEF through the Center for Cell Signaling & Drug Discovery Ewha Womans University .

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Expression of the cell surface adhesion receptor syndecan-2 is known to be involved in the regulation of cancer cell migration. However, the molecular mechanism of syndecan-2-mediated cell migration remains unknown. Here we report that Rac contributes to the regulation of syndecan-2-mediated cancer cell migration. Overexpression of syndecan-2 enhanced migration and invasion of human colon adenocarcinoma cells Caco-2 and HCT116 cells. In parallel with the increased cell migration/invasion, syndecan-2 overexpression enhanced Rac activity, while dominant negative Rac (RacN17) diminished syndecan-2-mediated increased cancer cell migration. In addition syndecan-2 expression increased membrane localization of Tiam1 and syndecan-2-mediated cell migration/invasion of Caco-2 cells was diminished when Tiam1 levels were knocked-down with small inhibitory RNAs. Furthermore, oligomerization-defective syndecan-2 mutants failed to increase membrane localization of Tiam1, activation of Rac and subsequent cell migration of both Caco-2 and HCT116 cells. Taken together, these results suggest that syndecan-2 regulates cell migration of colon carcinoma cells through Tiam1-dependent Rac activation in colon cancer cells.

AB - Expression of the cell surface adhesion receptor syndecan-2 is known to be involved in the regulation of cancer cell migration. However, the molecular mechanism of syndecan-2-mediated cell migration remains unknown. Here we report that Rac contributes to the regulation of syndecan-2-mediated cancer cell migration. Overexpression of syndecan-2 enhanced migration and invasion of human colon adenocarcinoma cells Caco-2 and HCT116 cells. In parallel with the increased cell migration/invasion, syndecan-2 overexpression enhanced Rac activity, while dominant negative Rac (RacN17) diminished syndecan-2-mediated increased cancer cell migration. In addition syndecan-2 expression increased membrane localization of Tiam1 and syndecan-2-mediated cell migration/invasion of Caco-2 cells was diminished when Tiam1 levels were knocked-down with small inhibitory RNAs. Furthermore, oligomerization-defective syndecan-2 mutants failed to increase membrane localization of Tiam1, activation of Rac and subsequent cell migration of both Caco-2 and HCT116 cells. Taken together, these results suggest that syndecan-2 regulates cell migration of colon carcinoma cells through Tiam1-dependent Rac activation in colon cancer cells.

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Syndecan-2 regulates cell migration in colon cancer cells through Tiam1-mediated Rac activation

Abstract

Keywords

UN SDGs

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