TY - JOUR
T1 - Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells
AU - Ryu, Heui Young
AU - Lee, Jiseon
AU - Yang, Sanghwa
AU - Park, Haein
AU - Choi, Sojoong
AU - Jung, Kyeong Cheon
AU - Lee, Seung Taek
AU - Seong, Je Kyung
AU - Han, Inn Oc
AU - Oh, Eok Soo
PY - 2009/12/18
Y1 - 2009/12/18
N2 - Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how syndecan-2 regulates colon cancer is unclear. In human colon adenocarcinoma tissue samples, we found that both mRNA and protein expression of syndecan-2 were increased, compared with the neighboring normal epithelium, suggesting that syndecan-2 plays functional roles in human colon cancer cells. Consistent with this notion, syndecan-2-overexpressing HT-29 colon adenocarcinoma cells showed enhanced migration/invasion, anchorage-independent growth, and primary tumor formation in nude mice, paralleling their morphological changes into highly tumorigenic cells. In addition, our experiments revealed that syndecan-2 enhanced both expression and secretion of matrix metalloproteinase-7 (MMP-7), directly interacted with pro-MMP-7, and potentiated the enzymatic activity of pro-MMP-7 by activating its processing into the active MMP-7. Collectively, these data strongly suggest that syndecan-2 functions as a docking receptor for pro-MMP-7 in colon cancer cells.
AB - Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how syndecan-2 regulates colon cancer is unclear. In human colon adenocarcinoma tissue samples, we found that both mRNA and protein expression of syndecan-2 were increased, compared with the neighboring normal epithelium, suggesting that syndecan-2 plays functional roles in human colon cancer cells. Consistent with this notion, syndecan-2-overexpressing HT-29 colon adenocarcinoma cells showed enhanced migration/invasion, anchorage-independent growth, and primary tumor formation in nude mice, paralleling their morphological changes into highly tumorigenic cells. In addition, our experiments revealed that syndecan-2 enhanced both expression and secretion of matrix metalloproteinase-7 (MMP-7), directly interacted with pro-MMP-7, and potentiated the enzymatic activity of pro-MMP-7 by activating its processing into the active MMP-7. Collectively, these data strongly suggest that syndecan-2 functions as a docking receptor for pro-MMP-7 in colon cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=72149109011&partnerID=8YFLogxK
U2 - 10.1074/jbc.M109.054254
DO - 10.1074/jbc.M109.054254
M3 - Article
C2 - 19858218
AN - SCOPUS:72149109011
SN - 0021-9258
VL - 284
SP - 35692
EP - 35701
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 51
ER -