TY - GEN
T1 - Synaptic connectivity of a low density patterned neuronal network produced on the poly-L-lysine stamped microelectrode array
AU - Jun, Sang Beom
AU - Hynd, Matthew
AU - Dowell-Mesfin, Natalie
AU - Smith, Karen L.
AU - Turner, James
AU - Shain, William
AU - Kim, Sung June
PY - 2005
Y1 - 2005
N2 - Rectangular networks of rat hippocampal neurons have been produced on microelectrode arrays (MEAs). The crossing points of networks were located at the recording electrode sites by aligned microcontact printing (μCP) technique. Polydimethysiloxane (PDMS) stamp was fabricated to print fine poly-L-Iysine (PLL) patterns of 2μm-width lines for neurites and 20μm-diameter circles for cell bodies. Different densities of neurons were applied on the PLL-stamped MEAs to find how a low density of neurons still has the functional connectivity. From the neural network applied with a density of 200 cells/mm2, we could observe signal propagation among spontaneous activities. Electrical responses were also evoked by 200μA current pulse stimulation with 50μs pulse width. Immunocytostaining was employed to identify dendrites, synapses, and nuclei in the patterned neurons.
AB - Rectangular networks of rat hippocampal neurons have been produced on microelectrode arrays (MEAs). The crossing points of networks were located at the recording electrode sites by aligned microcontact printing (μCP) technique. Polydimethysiloxane (PDMS) stamp was fabricated to print fine poly-L-Iysine (PLL) patterns of 2μm-width lines for neurites and 20μm-diameter circles for cell bodies. Different densities of neurons were applied on the PLL-stamped MEAs to find how a low density of neurons still has the functional connectivity. From the neural network applied with a density of 200 cells/mm2, we could observe signal propagation among spontaneous activities. Electrical responses were also evoked by 200μA current pulse stimulation with 50μs pulse width. Immunocytostaining was employed to identify dendrites, synapses, and nuclei in the patterned neurons.
UR - http://www.scopus.com/inward/record.url?scp=33846904800&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33846904800
SN - 0780387406
SN - 9780780387409
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 7604
EP - 7607
BT - Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
T2 - 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Y2 - 1 September 2005 through 4 September 2005
ER -