Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays

Byoung Hoon Kang; Youngseop Lee; Jinhyo Kim; Minhee Kang; Luke P. Lee; Ki Hun Jeong

Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays

Byoung Hoon Kang, Youngseop Lee, Jinhyo Kim, Minhee Kang, Luke P. Lee, Ki Hun Jeong

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

An amplification and SERS-based quantification of genetic molecules have been demonstrated for ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. Glass nanopillar arrays with gold nanoislands have multiple roles of plasmonic heat generator using photothermal effect as well as SERS substrate using local field enhancement. The photothermal heating by plasmonic nanopillar arrays on LED enables a ultrafast rate of thermal change with highly efficient amplification and the nanogap-rich structure supports a significant increase of SERS peak for conjugation between SYBR green and amplicon with low cycle threshold. This novel method can open up for improved point-of-care molecular diagnosis.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Chemical and Biological Microsystems Society
Pages	937-939
Number of pages	3
ISBN (Electronic)	9781510897571
State	Published - 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 11 Nov 2018 → 15 Nov 2018

Publication series

Name	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume	2

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/Territory	Taiwan, Province of China
City	Kaohsiung
Period	11/11/18 → 15/11/18

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2018018122, 2018029899).

Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

Keywords

Molecular diagnosis
Photothermal PCR
Surface-enhanced Raman scattering

Cite this

Kang, B. H., Lee, Y., Kim, J., Kang, M., Lee, L. P., & Jeong, K. H. (2018). Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 937-939). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 2). Chemical and Biological Microsystems Society.

Kang, Byoung Hoon ; Lee, Youngseop ; Kim, Jinhyo et al. / Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. pp. 937-939 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

@inproceedings{0427eed381db4666bee746d92e4db645,

title = "Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays",

abstract = "An amplification and SERS-based quantification of genetic molecules have been demonstrated for ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. Glass nanopillar arrays with gold nanoislands have multiple roles of plasmonic heat generator using photothermal effect as well as SERS substrate using local field enhancement. The photothermal heating by plasmonic nanopillar arrays on LED enables a ultrafast rate of thermal change with highly efficient amplification and the nanogap-rich structure supports a significant increase of SERS peak for conjugation between SYBR green and amplicon with low cycle threshold. This novel method can open up for improved point-of-care molecular diagnosis.",

keywords = "Molecular diagnosis, Photothermal PCR, Surface-enhanced Raman scattering",

author = "Kang, {Byoung Hoon} and Youngseop Lee and Jinhyo Kim and Minhee Kang and Lee, {Luke P.} and Jeong, {Ki Hun}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2018018122, 2018029899). Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

language = "English",

series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

pages = "937--939",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

}

Kang, BH, Lee, Y, Kim, J, Kang, M, Lee, LP & Jeong, KH 2018, Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, vol. 2, Chemical and Biological Microsystems Society, pp. 937-939, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, Taiwan, Province of China, 11/11/18.

Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. / Kang, Byoung Hoon; Lee, Youngseop; Kim, Jinhyo et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. p. 937-939 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays

AU - Kang, Byoung Hoon

AU - Lee, Youngseop

AU - Kim, Jinhyo

AU - Kang, Minhee

AU - Lee, Luke P.

AU - Jeong, Ki Hun

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2018018122, 2018029899). Publisher Copyright: Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

PY - 2018

Y1 - 2018

N2 - An amplification and SERS-based quantification of genetic molecules have been demonstrated for ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. Glass nanopillar arrays with gold nanoislands have multiple roles of plasmonic heat generator using photothermal effect as well as SERS substrate using local field enhancement. The photothermal heating by plasmonic nanopillar arrays on LED enables a ultrafast rate of thermal change with highly efficient amplification and the nanogap-rich structure supports a significant increase of SERS peak for conjugation between SYBR green and amplicon with low cycle threshold. This novel method can open up for improved point-of-care molecular diagnosis.

AB - An amplification and SERS-based quantification of genetic molecules have been demonstrated for ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. Glass nanopillar arrays with gold nanoislands have multiple roles of plasmonic heat generator using photothermal effect as well as SERS substrate using local field enhancement. The photothermal heating by plasmonic nanopillar arrays on LED enables a ultrafast rate of thermal change with highly efficient amplification and the nanogap-rich structure supports a significant increase of SERS peak for conjugation between SYBR green and amplicon with low cycle threshold. This novel method can open up for improved point-of-care molecular diagnosis.

KW - Molecular diagnosis

KW - Photothermal PCR

KW - Surface-enhanced Raman scattering

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M3 - Conference contribution

AN - SCOPUS:85079833925

T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

SP - 937

EP - 939

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Y2 - 11 November 2018 through 15 November 2018

ER -

Kang BH, Lee Y, Kim J, Kang M, Lee LP, Jeong KH. Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society. 2018. p. 937-939. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

Surface-enhanced Raman spectroscopy based ultrafast DNA assays using photothermal PCR chip with plasmonic nanopillar arrays

Abstract

Publication series

Conference

Bibliographical note

Keywords

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Cite this