Suppression of splenic lymphocyte proliferation by eucommia ulmoides and genipin

Gabsik Yang, Eun Kyoung Seo, Je Hyun Lee, Joo Young Lee

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16 Scopus citations


We investigated the modulation of innate and adaptive immune cell activation by Eucommia ulmoides Oliver extract (EUE) and its ingredient genipin. As an innate immunity indicator, the phagocytic activity of macrophages was determined by measuring engulfed, fluorescently labeled Escherichia coli. As a surrogate marker for the respective activation of cellular and humoral adaptive immunity, concanavalin A (ConA) and lipopolysaccharide (LPS) induction of primary splenocyte proliferation was assayed in in vitro and ex vivo systems. EUE and genipin suppressed the proliferation of primary splenic lymphocytes induced by ConA or LPS, but not macrophage phagocytosis. Oral administration of EUE and genipin to mice decreased splenic lymphocyte proliferation induced by ConA or LPS. These results revealed that E. ulmoides and genipin suppressed cellular and humoral adaptive immunity, and they suggest that E. ulmoides and genipin are promising candidates for immunosuppressive drugs that target diseases that involve excessive activation of adaptive immunity.

Original languageEnglish
Pages (from-to)538-546
Number of pages9
JournalChemistry and Biodiversity
Issue number4
StatePublished - 1 Apr 2015

Bibliographical note

Publisher Copyright:
Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.


  • Cellular immunity
  • Humoral immunity
  • Immunosuppression
  • Lymphocytes splenic
  • Macrophages


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