Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity

Su Hyun Song; Hye Young Min; Ah Reum Han; Joo Won Nam; Eun Kyoung Seo; Woo Park Seoung Woo Park; Hyung Lee Sang Hyung Lee; Kook Lee Sang Kook Lee

doi:10.1016/j.intimp.2008.12.003

Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity

Su Hyun Song, Hye Young Min, Ah Reum Han, Joo Won Nam, Eun Kyoung Seo, Woo Park Seoung Woo Park, Hyung Lee Sang Hyung Lee, Kook Lee Sang Kook Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Eleutherine americana Merr. et Heyne (Iridaceae) has been used as a folk medicine for the treatment of coronary disorders and wound-healing. In our previous phytochemical study, several pyranonaphthoquinoids, including (-)-isoeleutherin, were isolated from the bulbs of E. americana. Because inhibitors of inducible nitric oxide synthase (iNOS) might be useful as anti-inflammatory agents, we investigated the potential of pyranonaphthoquinoids to inhibit iNOS activity. We found that (-)-isoeleutherin inhibits lipopolysaccharide (LPS)-stimulated induction of nitric oxide (NO) in a dose-dependent manner (IC₅₀ = 7.4 μM). Using western blots and reverse transcription-polymerase chain reaction, we showed that (-)-isoeleutherin suppresses the expression of iNOS protein and mRNA. In addition, (-)-isoeleutherin inhibited the expression of various cytokines such as interleukin-1β and interferon-β. Activation of the transcriptional activity of NF-κB by LPS was also inhibited by treatment with (-)-isoeleutherin, suggesting that (-)-isoeleutherin-mediated suppression of iNOS expression is associated with the regulation of transcription factor NF-κB. These findings suggest that (-)-isoeleutherin might be a novel anti-inflammatory agent, and that it may work by inhibiting NFkB activation in macrophages.

Original language	English
Pages (from-to)	298-302
Number of pages	5
Journal	International Immunopharmacology
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/j.intimp.2008.12.003
State	Published - Mar 2009

Bibliographical note

Funding Information:
This work was supported by grant no. R15-2006-020 from the National Core Research Center (NCRC) program of the Ministry of Education, Science & Technology (MEST) and the Korea Science and Engineering Foundation (KOSEF) through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University.

Keywords

(-)-Isoeleutherin
Anti-inflammatory
Eleutherine americana
Inducible nitric oxide synthase (iNOS)
Nitric oxide (NO)
Nuclear factor kappa beta (NF-κB)

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10.1016/j.intimp.2008.12.003

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Song, S. H., Min, H. Y., Han, A. R., Nam, J. W., Seo, E. K., Seoung Woo Park, W. P., Sang Hyung Lee, H. L., & Sang Kook Lee, K. L. (2009). Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity. International Immunopharmacology, 9(3), 298-302. https://doi.org/10.1016/j.intimp.2008.12.003

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title = "Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity",

abstract = "Eleutherine americana Merr. et Heyne (Iridaceae) has been used as a folk medicine for the treatment of coronary disorders and wound-healing. In our previous phytochemical study, several pyranonaphthoquinoids, including (-)-isoeleutherin, were isolated from the bulbs of E. americana. Because inhibitors of inducible nitric oxide synthase (iNOS) might be useful as anti-inflammatory agents, we investigated the potential of pyranonaphthoquinoids to inhibit iNOS activity. We found that (-)-isoeleutherin inhibits lipopolysaccharide (LPS)-stimulated induction of nitric oxide (NO) in a dose-dependent manner (IC50 = 7.4 μM). Using western blots and reverse transcription-polymerase chain reaction, we showed that (-)-isoeleutherin suppresses the expression of iNOS protein and mRNA. In addition, (-)-isoeleutherin inhibited the expression of various cytokines such as interleukin-1β and interferon-β. Activation of the transcriptional activity of NF-κB by LPS was also inhibited by treatment with (-)-isoeleutherin, suggesting that (-)-isoeleutherin-mediated suppression of iNOS expression is associated with the regulation of transcription factor NF-κB. These findings suggest that (-)-isoeleutherin might be a novel anti-inflammatory agent, and that it may work by inhibiting NFkB activation in macrophages.",

keywords = "(-)-Isoeleutherin, Anti-inflammatory, Eleutherine americana, Inducible nitric oxide synthase (iNOS), Nitric oxide (NO), Nuclear factor kappa beta (NF-κB)",

author = "Song, {Su Hyun} and Min, {Hye Young} and Han, {Ah Reum} and Nam, {Joo Won} and Seo, {Eun Kyoung} and {Seoung Woo Park}, {Woo Park} and {Sang Hyung Lee}, {Hyung Lee} and {Sang Kook Lee}, {Kook Lee}",

note = "Funding Information: This work was supported by grant no. R15-2006-020 from the National Core Research Center (NCRC) program of the Ministry of Education, Science & Technology (MEST) and the Korea Science and Engineering Foundation (KOSEF) through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University.",

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Song, SH, Min, HY, Han, AR, Nam, JW, Seo, EK, Seoung Woo Park, WP, Sang Hyung Lee, HL & Sang Kook Lee, KL 2009, 'Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity', International Immunopharmacology, vol. 9, no. 3, pp. 298-302. https://doi.org/10.1016/j.intimp.2008.12.003

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AU - Song, Su Hyun

AU - Min, Hye Young

AU - Han, Ah Reum

AU - Nam, Joo Won

AU - Seo, Eun Kyoung

AU - Seoung Woo Park, Woo Park

AU - Sang Hyung Lee, Hyung Lee

AU - Sang Kook Lee, Kook Lee

N1 - Funding Information: This work was supported by grant no. R15-2006-020 from the National Core Research Center (NCRC) program of the Ministry of Education, Science & Technology (MEST) and the Korea Science and Engineering Foundation (KOSEF) through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University.

PY - 2009/3

Y1 - 2009/3

N2 - Eleutherine americana Merr. et Heyne (Iridaceae) has been used as a folk medicine for the treatment of coronary disorders and wound-healing. In our previous phytochemical study, several pyranonaphthoquinoids, including (-)-isoeleutherin, were isolated from the bulbs of E. americana. Because inhibitors of inducible nitric oxide synthase (iNOS) might be useful as anti-inflammatory agents, we investigated the potential of pyranonaphthoquinoids to inhibit iNOS activity. We found that (-)-isoeleutherin inhibits lipopolysaccharide (LPS)-stimulated induction of nitric oxide (NO) in a dose-dependent manner (IC50 = 7.4 μM). Using western blots and reverse transcription-polymerase chain reaction, we showed that (-)-isoeleutherin suppresses the expression of iNOS protein and mRNA. In addition, (-)-isoeleutherin inhibited the expression of various cytokines such as interleukin-1β and interferon-β. Activation of the transcriptional activity of NF-κB by LPS was also inhibited by treatment with (-)-isoeleutherin, suggesting that (-)-isoeleutherin-mediated suppression of iNOS expression is associated with the regulation of transcription factor NF-κB. These findings suggest that (-)-isoeleutherin might be a novel anti-inflammatory agent, and that it may work by inhibiting NFkB activation in macrophages.

AB - Eleutherine americana Merr. et Heyne (Iridaceae) has been used as a folk medicine for the treatment of coronary disorders and wound-healing. In our previous phytochemical study, several pyranonaphthoquinoids, including (-)-isoeleutherin, were isolated from the bulbs of E. americana. Because inhibitors of inducible nitric oxide synthase (iNOS) might be useful as anti-inflammatory agents, we investigated the potential of pyranonaphthoquinoids to inhibit iNOS activity. We found that (-)-isoeleutherin inhibits lipopolysaccharide (LPS)-stimulated induction of nitric oxide (NO) in a dose-dependent manner (IC50 = 7.4 μM). Using western blots and reverse transcription-polymerase chain reaction, we showed that (-)-isoeleutherin suppresses the expression of iNOS protein and mRNA. In addition, (-)-isoeleutherin inhibited the expression of various cytokines such as interleukin-1β and interferon-β. Activation of the transcriptional activity of NF-κB by LPS was also inhibited by treatment with (-)-isoeleutherin, suggesting that (-)-isoeleutherin-mediated suppression of iNOS expression is associated with the regulation of transcription factor NF-κB. These findings suggest that (-)-isoeleutherin might be a novel anti-inflammatory agent, and that it may work by inhibiting NFkB activation in macrophages.

KW - (-)-Isoeleutherin

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KW - Eleutherine americana

KW - Inducible nitric oxide synthase (iNOS)

KW - Nitric oxide (NO)

KW - Nuclear factor kappa beta (NF-κB)

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AN - SCOPUS:60249085139

SN - 1567-5769

VL - 9

SP - 298

EP - 302

JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 3

ER -

Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity

Abstract

Bibliographical note

Keywords

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