Study on the mechanisms of seizure-like events suppression effect by electrical stimulation using a microelectrode array

Sora Ahn, Sumin Jo, Sang Beom Jun, Hyang Woon Lee, Seungjun Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


In this paper, we studied the mechanisms underlying thesuppression of seizure-like events (SLEs) by electricalstimulation. We conducted an in-vitro experiment using entorhinal cortex combined hippocampal slices and two convulsant drugs, bicuculline and 4-aminopyridine, to induce spontaneous SLEs. We used a microelectrode array to observe network dynamics over the entire hippocampal area simultaneously, including regions far from the stimulation site. We stimulated the entorhinal cortex region, which has been determined to be a focus of SLEs by Granger causality analysis of multichannel time series data, by an external electrode. In bicuculline application, electrical stimulation showed a marked suppression effect, even though the sizes of the effective region differed. In 4-aminopyridine application, however, stimulation under the same conditions did not suppress the activities in ~80% of SLEs. The suppression effect was more remarkable in the areas surrounding the stimulation site in both cases. Our experimental results could support the neuronal depolarization blockade mechanism by accumulation of extracellular potassium ions, which is one of the most convincing mechanisms to understand seizure suppression phenomena because of electrical stimulation. Computer simulation using a neuronal network model also confirmed the mechanism.

Original languageEnglish
Pages (from-to)471-478
Number of pages8
Issue number9
StatePublished - 1 May 2017

Bibliographical note

Publisher Copyright:
© Copyright 2017 Wolters Kluwer Health, Inc. All rights reserved.


  • Granger causality
  • depolarization blockade
  • in-vitro experiment
  • microelectrode array
  • potassium accumulation hypothesis
  • seizure-like event
  • temporal lobe epilepsy


Dive into the research topics of 'Study on the mechanisms of seizure-like events suppression effect by electrical stimulation using a microelectrode array'. Together they form a unique fingerprint.

Cite this