The Rev binding to Rev Responsive Element (RRE) of HIV-1 mRNA plays an important role in the HIV-1 viral replication cycle. The disruption of the Rev-RRE interaction has been studied extensively in order to develop a potential antiviral drug. In order to provide the basis for a more promising approach to develop a Rev-RRE binding inhibitor against HIV-1 infection, it is necessary to understand the binding modes of the aminoglycoside antibiotics to RRE. In the present study, the binding mode of a modified antibiotic, a neamine conjugated with pyrene and arginine (NCPA), to RRE has been studied by the methods of Tm measurement and spectroscopic analysis of RRE with or without antibiotics. The results confirmed that NCPA competes with Rev in binding to RRE.
|Number of pages
|Journal of Microbiology and Biotechnology
|Published - Jan 2006
- Aminoglycoside antibiotics
- Binding affinity to RRE
- HIV-1 RRE
- Inhibition of HIV-1 Rev-RRE interaction