Structural radial heterogeneity of a silica-based wide-bore monolithic column

Khaled S. Mriziq, Jude A. Abia, Youngmi Lee, Georges Guiochon

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54 Scopus citations


The radial distribution of the main characteristics (elution time and standard deviation) of the elution profiles of a flat injected band recorded at the exit of a monolithic column were determined. These distributions provide the radial distributions of the average mobile phase velocity, the elution time and the maximum height of the peak of an analyte, the column efficiency and the analyte concentration. The band profiles were measured at the exit of a 10-mm i.d., 100-mm long silica-based monolithic column. An on-column local electrochemical amperometric detector allowed the recording of the elution profiles at different spatial positions throughout the column cross-section. The local spatial distribution of the mobile phase velocity does not follow a piston-flow behavior but exhibits radial heterogeneity. The local efficiency near the wall is lower than that near the column center. The radial distribution of the maximum concentration of the peaks varies throughout the column exit section, partially due to the radial variations of the column efficiency. These results might explain the rather large value of the A term of the Van Deemter or the Knox equations reported previously for monolithic columns.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalJournal of Chromatography A
Issue number1-2
StatePublished - 6 Jun 2008

Bibliographical note

Funding Information:
This work was supported in part by Grant DE-FG05-88-ER-13869 of the US Department of Energy and by the cooperative agreement between the University of Tennessee and Oak Ridge National Laboratory.


  • Column efficiency
  • Monolithic columns
  • Radial column homogeneity


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