TY - JOUR
T1 - Structural genomics of minimal organisms
T2 - pipeline and results.
AU - Kim, Sung Hou
AU - Shin, Dong Hae
AU - Kim, Rosalind
AU - Adams, Paul
AU - Chandonia, John Marc
PY - 2008
Y1 - 2008
N2 - The initial objective of the Berkeley Structural Genomics Center was to obtain a near complete three-dimensional (3D) structural information of all soluble proteins of two minimal organisms, closely related pathogens Mycoplasma genitalium and M. pneumoniae. The former has fewer than 500 genes and the latter has fewer than 700 genes. A semiautomated structural genomics pipeline was set up from target selection, cloning, expression, purification, and ultimately structural determination. At the time of this writing, structural information of more than 93% of all soluble proteins of M. genitalium is avail able. This chapter summarizes the approaches taken by the authors' center.
AB - The initial objective of the Berkeley Structural Genomics Center was to obtain a near complete three-dimensional (3D) structural information of all soluble proteins of two minimal organisms, closely related pathogens Mycoplasma genitalium and M. pneumoniae. The former has fewer than 500 genes and the latter has fewer than 700 genes. A semiautomated structural genomics pipeline was set up from target selection, cloning, expression, purification, and ultimately structural determination. At the time of this writing, structural information of more than 93% of all soluble proteins of M. genitalium is avail able. This chapter summarizes the approaches taken by the authors' center.
UR - http://www.scopus.com/inward/record.url?scp=49649114450&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-058-8_32
DO - 10.1007/978-1-60327-058-8_32
M3 - Article
C2 - 18542885
AN - SCOPUS:49649114450
SN - 1064-3745
VL - 426
SP - 475
EP - 496
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -