Structural genomics of minimal organisms: pipeline and results.

Sung Hou Kim, Dong Hae Shin, Rosalind Kim, Paul Adams, John Marc Chandonia

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The initial objective of the Berkeley Structural Genomics Center was to obtain a near complete three-dimensional (3D) structural information of all soluble proteins of two minimal organisms, closely related pathogens Mycoplasma genitalium and M. pneumoniae. The former has fewer than 500 genes and the latter has fewer than 700 genes. A semiautomated structural genomics pipeline was set up from target selection, cloning, expression, purification, and ultimately structural determination. At the time of this writing, structural information of more than 93% of all soluble proteins of M. genitalium is avail able. This chapter summarizes the approaches taken by the authors' center.

Original languageEnglish
Pages (from-to)475-496
Number of pages22
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2008


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