Abstract
Background: Ovarian tumor domain-containing protein 1 (OTU1) acts as a deubiquitinating enzyme in the endoplasmic reticulum-associated degradation (ERAD) pathway by associating with valosin-containing protein (VCP). Results: One OTU1 binds to a VCP hexamer with a KD of 0.71 M. Conclusion: The 39GYPP42 (S3/S4) loop of OTU1 is critical for interaction with VCP. Significance: This is the first structural study of VCP and OTU1 complex.
Original language | English |
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Pages (from-to) | 12264-12274 |
Number of pages | 11 |
Journal | Journal of Biological Chemistry |
Volume | 289 |
Issue number | 18 |
DOIs | |
State | Published - 2014 |