Structural and functional alterations at pre-epileptic stage are closely associated with epileptogenesis in pilocarpine-induced epilepsy model

Hani Kim, Yunsook Choi, Hye Young Joung, Yun Seo Choi, Hyeon Jin Kim, Yohan Joo, Jin Hwan Oh, Hoo Jae Hann, Zang Hee Cho, Hyang Woon Lee

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9 Scopus citations


Pilocarpine-induced rat epilepsy model is an established animal model that mimics medial temporal lobe epilepsy in humans. The purpose of this study was to investigate neuroimaging abnormalities in various stages of epileptogenesis and to correlate them with seizure severity in pilocarpine-induced rat epilepsy model. Fifty male Sprague-Dawley rats were subject to continuous video and electroencephalographic monitoring after inducing status epilepticus (SE) and seizure severity was estimated by frequency and total durations of class 3 to 5 spontaneous recurrent seizures (SRS) by modified Racine's classification. The 7.0 Tesla magnetic resonance imaging (MRI) with high resolution flurodeoxyglucose positron emission tomography (FDG-PET) was performed at 3 hours, 1, 3, 7 days and 4 weeks after the initial insult. The initial SRS was observed 9.7±1.3 days after the pilocarpine injection. MRI revealed an abnormal T2 signal change with swelling in both hippocampi and amygdala in acute (day 1 after injection) and latent phases (days 3 and 7), in association with PET hypometabolism in these areas. Interestingly, the mean frequency of class 3 to 5 SRS was positively correlated with abnormal T2 signals in hippocampal area at 3 days. SRS duration became longer with more decreased glucose metabolism in both hippocampi and amygdala at 7 days after pilocarpine injection. This study indicates that development and severity of SRS at chronic phase could be closely related with structural and functional changes in hippocampus during the latent period, a pre-epileptic stage.

Original languageEnglish
Pages (from-to)287-294
Number of pages8
JournalExperimental Neurobiology
Issue number5
StatePublished - 1 Oct 2017

Bibliographical note

Funding Information:
This work was supported by grants of the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2014R1A2A1A11052103 and 2017R1A2A2A05069647), and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare (HI14C1989) to H.W. Lee.

Publisher Copyright:
© Experimental Neurobiology 2017.


  • Epileptogenesis
  • MRI
  • Pilocarpine
  • Temporal lobe epilepsy


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