Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS)

Ivan K. Dimov, Lourdes Basabe-Desmonts, Jose L. Garcia-Cordero, Benjamin M. Ross, Antonio J. Ricco, Luke P. Lee

Research output: Contribution to journalArticlepeer-review

295 Scopus citations

Abstract

We present a self-powered integrated microfluidic blood analysis system (SIMBAS) that does not require any external connections, tethers, or tubing to deliver and analyze a raw whole-blood sample. SIMBAS only requires the user to place a 5 μL droplet of whole-blood at the inlet port of the device, whereupon the stand-alone SIMBAS performs on-chip removal of red and white cells, without external valving or pumping mechanisms, followed by analyte detection in platelet-containing plasma. Five complete biotin-streptavidin sample-to-answer assays are performed in 10 min; the limit of detection is 1.5 pM. Red and white blood cells are removed by trapping them in an integral trench structure. Simulations and experimental data show 99.9% to 100% blood cell retention in the passive structure. Powered by pre-evacuation of its PDMS substrate, SIMBAS' guiding design principle is the integration of the minimal number of components without sacrificing effectiveness in performing rapid complete bioassays, a critical step towards point-of-care molecular diagnostics.

Original languageEnglish
Pages (from-to)845-850
Number of pages6
JournalLab on a Chip
Volume11
Issue number5
DOIs
StatePublished - 7 Mar 2011

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