STA10 repression of STA gene expression is caused by a defective activator, flo8, in Saccharomyces cerevisiae

Tae Soo Kim, Ji Yeon Ahn, Jin Ho Yoon, Hyen Sam Kang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The expression of STA genes that encode extracellular glucoamylase isozymes is repressed in most laboratory Saccharomyces cerevisiae strains, which are believed to contain an undefined repressor, designated STA10. To identify the regulator involved in STA10 repression, we investigate the FLO8, MSN1, MSS11, STE12, and TEC1 genes. The Δflo8 or Δmss11 deletion mutants in the sta10 genetic background exhibit both a loss of flocculation ability and a reduction in extracellular glucoamylase activity, as in the STA10 strain. Moreover, the STA10 repression is suppressed completely or partially by the introduction of a single copy of the FLO8 or MSS11 genes. Sequence analysis and complementation testing of the STA10 strain reveal that it has an inactive, mutated flo8-1 allele. A random spore analysis and transplacement (allele replacement) experiment confirms that the repressive phenotype of STA10 is due to the amber mutation of the transcriptional activator, FLO8.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalCurrent Genetics
Volume44
Issue number5
DOIs
StatePublished - Dec 2003

Keywords

  • S. cerevisiae
  • STA gene
  • STA10 repression
  • Transcriptional activator FLO8

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