@article{de15e9cafb814884b0573e4fb61cc0db,
title = "SPSB1 enhances ovarian cancer cell survival by destabilizing p21",
abstract = "SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein with unknown functions in cancer cells. In this study, we found that SPSB1 knockdown markedly decreased the viability and migration of ovarian cancer cells, while ectopic SPSB1 overexpression in IL-3-dependent Ba/F3 cells significantly increased their proliferation rate compared with empty vector-transfected cells. SPSB1 knockdown significantly elevated p21 protein and mRNA levels and induced apoptosis in ovarian cancer cells, as evidenced by increased levels of cleaved PARP and decreased levels of Bcl-2. Notably, mechanistic investigations revealed that SPSB1 accelerated p21 destabilization by directly interacting with p21 and promoting its ubiquitin-mediated proteasomal degradation. Taken together, our findings provide novel insights into the role of SPSB1 in ovarian cancer cells.",
keywords = "Cell survival, Ovarian cancer, SPSB1, Ubiquitination, p21 destabilization",
author = "Kim, {Hyun Jung} and Kim, {Hye Jin} and Kim, {Mi Kyung} and Bae, {Moon Kyoung} and Sung, {Hye Youn} and Ahn, {Jung Hyuck} and Kim, {Yun Hwan} and Kim, {Seung Cheol} and Woong Ju",
note = "Funding Information: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2017R1A2B4002320), the Korean Health Technology R&D Project of the Ministry of Health and Welfare(HI12C0050), and RP-Grant 2018 of Ewha Womans University. We thank James P. Mahaffey, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript. Funding Information: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF- 2017R1A2B4002320 ), the Korean Health Technology R&D Project of the Ministry of Health and Welfare ( HI12C0050 ), and RP-Grant 2018 of Ewha Womans University . We thank James P. Mahaffey, PhD, from Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = mar,
day = "12",
doi = "10.1016/j.bbrc.2019.01.088",
language = "English",
volume = "510",
pages = "364--369",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",
}