SPSB1 enhances ovarian cancer cell survival by destabilizing p21

Hyun Jung Kim, Hye Jin Kim, Mi Kyung Kim, Moon Kyoung Bae, Hye Youn Sung, Jung Hyuck Ahn, Yun Hwan Kim, Seung Cheol Kim, Woong Ju

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein with unknown functions in cancer cells. In this study, we found that SPSB1 knockdown markedly decreased the viability and migration of ovarian cancer cells, while ectopic SPSB1 overexpression in IL-3-dependent Ba/F3 cells significantly increased their proliferation rate compared with empty vector-transfected cells. SPSB1 knockdown significantly elevated p21 protein and mRNA levels and induced apoptosis in ovarian cancer cells, as evidenced by increased levels of cleaved PARP and decreased levels of Bcl-2. Notably, mechanistic investigations revealed that SPSB1 accelerated p21 destabilization by directly interacting with p21 and promoting its ubiquitin-mediated proteasomal degradation. Taken together, our findings provide novel insights into the role of SPSB1 in ovarian cancer cells.

Original languageEnglish
Pages (from-to)364-369
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - 12 Mar 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Inc.


  • Cell survival
  • Ovarian cancer
  • SPSB1
  • Ubiquitination
  • p21 destabilization


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