Spatially targeted stimulation of cells for quantitative Gap Junction analysis

Sisi Chen; Luke Lee

Spatially targeted stimulation of cells for quantitative Gap Junction analysis

Department of Chemistry & Nanoscience

Research output: Contribution to conference › Paper › peer-review

Abstract

We present here a microfluidic device for studying Gap Junction Intercellular Communication (GJIC) by hydrodynamically focusing dye molecules over a confluent monolayer of cells cultured in 50-μm channels. The property of laminar flow ensures that dye molecules are focused over a spatially targeted line of cells, while leaving their neighboring cells undisturbed. The presence and extent of dye transfer can then be assessed using a time-lapse fluorescence microscopy.

Original language	English
Pages	1931-1933
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Cell-cell communication
Dye transfer
Gap junction
Hydrodynamic focusing

Cite this

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title = "Spatially targeted stimulation of cells for quantitative Gap Junction analysis",

abstract = "We present here a microfluidic device for studying Gap Junction Intercellular Communication (GJIC) by hydrodynamically focusing dye molecules over a confluent monolayer of cells cultured in 50-μm channels. The property of laminar flow ensures that dye molecules are focused over a spatially targeted line of cells, while leaving their neighboring cells undisturbed. The presence and extent of dye transfer can then be assessed using a time-lapse fluorescence microscopy.",

keywords = "Cell-cell communication, Dye transfer, Gap junction, Hydrodynamic focusing",

author = "Sisi Chen and Luke Lee",

year = "2008",

language = "English",

pages = "1931--1933",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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T1 - Spatially targeted stimulation of cells for quantitative Gap Junction analysis

AU - Chen, Sisi

AU - Lee, Luke

PY - 2008

Y1 - 2008

N2 - We present here a microfluidic device for studying Gap Junction Intercellular Communication (GJIC) by hydrodynamically focusing dye molecules over a confluent monolayer of cells cultured in 50-μm channels. The property of laminar flow ensures that dye molecules are focused over a spatially targeted line of cells, while leaving their neighboring cells undisturbed. The presence and extent of dye transfer can then be assessed using a time-lapse fluorescence microscopy.

AB - We present here a microfluidic device for studying Gap Junction Intercellular Communication (GJIC) by hydrodynamically focusing dye molecules over a confluent monolayer of cells cultured in 50-μm channels. The property of laminar flow ensures that dye molecules are focused over a spatially targeted line of cells, while leaving their neighboring cells undisturbed. The presence and extent of dye transfer can then be assessed using a time-lapse fluorescence microscopy.

KW - Cell-cell communication

KW - Dye transfer

KW - Gap junction

KW - Hydrodynamic focusing

UR - http://www.scopus.com/inward/record.url?scp=84902497965&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902497965

SP - 1931

EP - 1933

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Spatially targeted stimulation of cells for quantitative Gap Junction analysis

Abstract

Conference

Keywords

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Cite this