Soft-state biomicrofluidic pulse generator for single cell analysis

Poorya Sabounchi; Cristian Ionescu-Zanetti; Roger Chen; Manjiree Karandikar; Jeonggi Seo; Luke P. Lee

doi:10.1063/1.2195106

Soft-state biomicrofluidic pulse generator for single cell analysis

Poorya Sabounchi, Cristian Ionescu-Zanetti, Roger Chen, Manjiree Karandikar, Jeonggi Seo, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

We present the design, fabrication, and characterization of a soft-state biomicrofluidic pulse generator for single cell analysis. Hydrodynamic cell trapping via lateral microfluidic junctions allows the trapping of single cells from a bulk suspension. Microfluidic injection sites adjacent to the cell-trapping channels enable the pulsed delivery of nanoliter volumes of biochemical reagent. We demonstrated the application and removal of reagent at a frequency of 10 Hz with a rise time of less than 33 ms and a reagent consumption rate of 0.2 nLs. It is shown that this system operates as a low-pass filter with a cutoff frequency of 7 Hz.

Original language	English
Article number	183901
Journal	Applied Physics Letters
Volume	88
Issue number	18
DOIs	https://doi.org/10.1063/1.2195106
State	Published - 1 May 2006

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abstract = "We present the design, fabrication, and characterization of a soft-state biomicrofluidic pulse generator for single cell analysis. Hydrodynamic cell trapping via lateral microfluidic junctions allows the trapping of single cells from a bulk suspension. Microfluidic injection sites adjacent to the cell-trapping channels enable the pulsed delivery of nanoliter volumes of biochemical reagent. We demonstrated the application and removal of reagent at a frequency of 10 Hz with a rise time of less than 33 ms and a reagent consumption rate of 0.2 nLs. It is shown that this system operates as a low-pass filter with a cutoff frequency of 7 Hz.",

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AU - Sabounchi, Poorya

AU - Ionescu-Zanetti, Cristian

AU - Chen, Roger

AU - Karandikar, Manjiree

AU - Seo, Jeonggi

AU - Lee, Luke P.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - We present the design, fabrication, and characterization of a soft-state biomicrofluidic pulse generator for single cell analysis. Hydrodynamic cell trapping via lateral microfluidic junctions allows the trapping of single cells from a bulk suspension. Microfluidic injection sites adjacent to the cell-trapping channels enable the pulsed delivery of nanoliter volumes of biochemical reagent. We demonstrated the application and removal of reagent at a frequency of 10 Hz with a rise time of less than 33 ms and a reagent consumption rate of 0.2 nLs. It is shown that this system operates as a low-pass filter with a cutoff frequency of 7 Hz.

AB - We present the design, fabrication, and characterization of a soft-state biomicrofluidic pulse generator for single cell analysis. Hydrodynamic cell trapping via lateral microfluidic junctions allows the trapping of single cells from a bulk suspension. Microfluidic injection sites adjacent to the cell-trapping channels enable the pulsed delivery of nanoliter volumes of biochemical reagent. We demonstrated the application and removal of reagent at a frequency of 10 Hz with a rise time of less than 33 ms and a reagent consumption rate of 0.2 nLs. It is shown that this system operates as a low-pass filter with a cutoff frequency of 7 Hz.

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VL - 88

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 18

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Soft-state biomicrofluidic pulse generator for single cell analysis

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