Abstract
We present the design, fabrication, and characterizations of an integrated soft-state biochemical pulse generator within single cell trapping device. Biochemical pulses are generated around trapped single cell array to study the kinetic response of membrane proteins. Hydrodynamic cell trapping via lateral channels allows the trapping of single cells from a bulk suspension. Microfluidic injection site adjacent to the cell-trapping channels enable the pulsed delivery of nanoliter volumes of biochemical reagent. Fast endocytic recycling of cells is studied under 0.5 Hz pulsed flow using a FM fluorescent dye and intensity results were compared to a simple compartment model.
| Original language | English |
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| Pages | 990-992 |
| Number of pages | 3 |
| State | Published - 2006 |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006 |
Conference
| Conference | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
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| Country/Territory | Japan |
| City | Tokyo |
| Period | 5/11/06 → 9/11/06 |
Keywords
- Biochemical pulses
- Single cell analysis
- Soft-state devices