TY - JOUR
T1 - Small Molecule Detection in Saliva Facilitates Portable Tests of Marijuana Abuse
AU - Lee, Jung Rok
AU - Choi, Joohong
AU - Shultz, Tyler O.
AU - Wang, Shan X.
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/8/2
Y1 - 2016/8/2
N2 - As medical and recreational use of cannabis, or marijuana, becomes more prevalent, law enforcement needs a tool to evaluate whether drivers are operating vehicles under the influence of cannabis, specifically the psychoactive substance, tetrahydrocannabinol (THC). However, the cutoff concentration of THC that causes impairment is still controversial, and current on-site screening tools are not sensitive enough to detect trace amounts of THC in oral fluids. Here we present a novel sensing platform that employs giant magnetoresistive (GMR) biosensors integrated with a portable reader system and smartphone to detect THC in saliva using competitive assays. With a simple saliva collection scheme, we have optimized the assay to measure THC in the range from 0 to 50 ng/mL, covering most cutoff values proposed in previous studies. This work facilitates on-site screening for THC and shows potential for testing of other small molecule drugs and analytes in point-of-care (POC) settings.
AB - As medical and recreational use of cannabis, or marijuana, becomes more prevalent, law enforcement needs a tool to evaluate whether drivers are operating vehicles under the influence of cannabis, specifically the psychoactive substance, tetrahydrocannabinol (THC). However, the cutoff concentration of THC that causes impairment is still controversial, and current on-site screening tools are not sensitive enough to detect trace amounts of THC in oral fluids. Here we present a novel sensing platform that employs giant magnetoresistive (GMR) biosensors integrated with a portable reader system and smartphone to detect THC in saliva using competitive assays. With a simple saliva collection scheme, we have optimized the assay to measure THC in the range from 0 to 50 ng/mL, covering most cutoff values proposed in previous studies. This work facilitates on-site screening for THC and shows potential for testing of other small molecule drugs and analytes in point-of-care (POC) settings.
UR - http://www.scopus.com/inward/record.url?scp=84982671767&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.6b01688
DO - 10.1021/acs.analchem.6b01688
M3 - Article
C2 - 27434697
AN - SCOPUS:84982671767
SN - 0003-2700
VL - 88
SP - 7457
EP - 7461
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 15
ER -