Small molecule activator of Nm23/NDPK as an inhibitor of metastasis

Jae Jin Lee, Hwang Suk Kim, Ji Sun Lee, Jimin Park, Sang Chul Shin, Soonwha Song, Eunsun Lee, Jung Eun Choi, Ji Wan Suh, Hongsoo Lee, Eunice Eun Kyeong Kim, Eun Kyoung Seo, Dong Hae Shin, Ho Young Lee, Hee Yoon Lee, Kong Joo Lee

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Nm23-H1/NDPK-A is a tumor metastasis suppressor having NDP kinase (NDPK) activity. Nm23-H1 is positively associated with prolonged disease-free survival and good prognosis of cancer patients. Approaches to increasing the cellular levels of Nm23-H1 therefore have significance in the therapy of metastatic cancers. We found a small molecule, (±)-trans-3-(3,4-dimethoxyphenyl)-4-[(E)-3,4-dimethoxystyryl]cyclohex-1-ene, that activates Nm23, hereafter called NMac1. NMac1 directly binds to Nm23-H1 and increases its NDPK activity. Employing various NMac1 derivatives and hydrogen/deuterium mass spectrometry (HDX-MS), we identified the pharmacophore and mode of action of NMac1. We found that NMac1 binds to the C-terminal of Nm23-H1 and induces the NDPK activation through its allosteric conformational changes. NMac1-treated MDA-MB-231 breast cancer cells showed dramatic changes in morphology and actin-cytoskeletal organization following inhibition of Rac1 activation. NMac1 also suppressed invasion and migration in vitro, and metastasis in vivo, in a breast cancer mouse model. NMac1 as an activator of NDPK has potential as an anti-metastatic agent.

Original languageEnglish
Article number10909
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - 1 Dec 2018

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