SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

Ji Seon Seo; Tae Kyung Kim; Yea Hyun Leem; Kang Woo Lee; Sun Kyu Park; In Sun Baek; Kyoung Shim Kim; Guang Jin Im; Soo Min Lee; Yang Hae Park; Pyung Lim Han

doi:10.1016/j.brainres.2009.01.019

SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

Ji Seon Seo
, Tae Kyung Kim
, Yea Hyun Leem
, Kang Woo Lee
, Sun Kyu Park
, In Sun Baek
, Kyoung Shim Kim
, Guang Jin Im
, Soo Min Lee
, Yang Hae Park
, Pyung Lim Han

Divison of Brain & Cognitive Sciences

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H₂O₂ in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.

Original language	English
Pages (from-to)	100-108
Number of pages	9
Journal	Brain Research
Volume	1261
DOIs	https://doi.org/10.1016/j.brainres.2009.01.019
State	Published - 19 Mar 2009

Bibliographical note

Funding Information:
This work was supported by a grant (A050580) from Korean health R&D project from Ministry of Health and Welfare, Republic of Korea.

Keywords

Alzheimer disease
Aβ
Natural compound
Plaque
ROS

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10.1016/j.brainres.2009.01.019

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@article{d1bdc6a67d2046a98593a33d8bd82bd1,

title = "SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice",

abstract = "SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66\% and 79\% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H2O2 in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.",

keywords = "Alzheimer disease, Aβ, Natural compound, Plaque, ROS",

author = "Seo, \{Ji Seon\} and Kim, \{Tae Kyung\} and Leem, \{Yea Hyun\} and Lee, \{Kang Woo\} and Park, \{Sun Kyu\} and Baek, \{In Sun\} and Kim, \{Kyoung Shim\} and Im, \{Guang Jin\} and Lee, \{Soo Min\} and Park, \{Yang Hae\} and Han, \{Pyung Lim\}",

note = "Funding Information: This work was supported by a grant (A050580) from Korean health R\&D project from Ministry of Health and Welfare, Republic of Korea. ",

year = "2009",

month = mar,

day = "19",

doi = "10.1016/j.brainres.2009.01.019",

language = "English",

volume = "1261",

pages = "100--108",

journal = "Brain Research",

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TY - JOUR

T1 - SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

AU - Seo, Ji Seon

AU - Kim, Tae Kyung

AU - Leem, Yea Hyun

AU - Lee, Kang Woo

AU - Park, Sun Kyu

AU - Baek, In Sun

AU - Kim, Kyoung Shim

AU - Im, Guang Jin

AU - Lee, Soo Min

AU - Park, Yang Hae

AU - Han, Pyung Lim

N1 - Funding Information: This work was supported by a grant (A050580) from Korean health R&D project from Ministry of Health and Welfare, Republic of Korea.

PY - 2009/3/19

Y1 - 2009/3/19

N2 - SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H2O2 in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.

AB - SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H2O2 in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.

KW - Alzheimer disease

KW - Aβ

KW - Natural compound

KW - Plaque

KW - ROS

UR - https://www.scopus.com/pages/publications/61849182567

U2 - 10.1016/j.brainres.2009.01.019

DO - 10.1016/j.brainres.2009.01.019

M3 - Article

C2 - 19401163

AN - SCOPUS:61849182567

SN - 0006-8993

VL - 1261

SP - 100

EP - 108

JO - Brain Research

JF - Brain Research

ER -

SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

Abstract

Bibliographical note

Keywords

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