SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

Ji Seon Seo; Tae Kyung Kim; Yea Hyun Leem; Kang Woo Lee; Sun Kyu Park; In Sun Baek; Kyoung Shim Kim; Guang Jin Im; Soo Min Lee; Yang Hae Park; Pyung Lim Han

doi:10.1016/j.brainres.2009.01.019

SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

Ji Seon Seo, Tae Kyung Kim, Yea Hyun Leem, Kang Woo Lee, Sun Kyu Park, In Sun Baek, Kyoung Shim Kim, Guang Jin Im, Soo Min Lee, Yang Hae Park, Pyung Lim Han

Divison of Brain & Cognitive Sciences

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H₂O₂ in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.

Original language	English
Pages (from-to)	100-108
Number of pages	9
Journal	Brain Research
Volume	1261
DOIs	https://doi.org/10.1016/j.brainres.2009.01.019
State	Published - 19 Mar 2009

Bibliographical note

Funding Information:
This work was supported by a grant (A050580) from Korean health R&D project from Ministry of Health and Welfare, Republic of Korea.

Keywords

Alzheimer disease
Aβ
Natural compound
Plaque
ROS

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10.1016/j.brainres.2009.01.019

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@article{d1bdc6a67d2046a98593a33d8bd82bd1,

title = "SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice",

abstract = "SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H2O2 in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.",

keywords = "Alzheimer disease, Aβ, Natural compound, Plaque, ROS",

author = "Seo, {Ji Seon} and Kim, {Tae Kyung} and Leem, {Yea Hyun} and Lee, {Kang Woo} and Park, {Sun Kyu} and Baek, {In Sun} and Kim, {Kyoung Shim} and Im, {Guang Jin} and Lee, {Soo Min} and Park, {Yang Hae} and Han, {Pyung Lim}",

note = "Funding Information: This work was supported by a grant (A050580) from Korean health R&D project from Ministry of Health and Welfare, Republic of Korea. ",

year = "2009",

month = mar,

day = "19",

doi = "10.1016/j.brainres.2009.01.019",

language = "English",

volume = "1261",

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journal = "Brain Research",

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T1 - SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

AU - Seo, Ji Seon

AU - Kim, Tae Kyung

AU - Leem, Yea Hyun

AU - Lee, Kang Woo

AU - Park, Sun Kyu

AU - Baek, In Sun

AU - Kim, Kyoung Shim

AU - Im, Guang Jin

AU - Lee, Soo Min

AU - Park, Yang Hae

AU - Han, Pyung Lim

N1 - Funding Information: This work was supported by a grant (A050580) from Korean health R&D project from Ministry of Health and Welfare, Republic of Korea.

PY - 2009/3/19

Y1 - 2009/3/19

N2 - SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H2O2 in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.

AB - SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H2O2 in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.

KW - Alzheimer disease

KW - Aβ

KW - Natural compound

KW - Plaque

KW - ROS

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JF - Brain Research

ER -

SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

Abstract

Bibliographical note

Keywords

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