Single-Step LRET Aptasensor for Rapid Mycotoxin Detection

Eun Jung Jo, Ju Young Byun, Hyoyoung Mun, Doyeon Bang, Jun Ho Son, Jae Young Lee, Luke P. Lee, Min Gon Kim

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Contamination of foods by mycotoxins is a common yet serious problem. Owing to the increase in consumption of fresh produce, consumers have become aware of food safety issues caused by mycotoxins. Therefore, rapid and sensitive mycotoxin detection is in great demand in fields such as food safety and public health. Here we report a single-step luminescence resonance energy transfer (LRET) aptasensor for mycotoxin detection. To accomplish the single-step sensor, our sensor was constructed by linking a quencher-labeled aptamer through a linker to the surface of upconversion nanoparticles (UCNPs). Our LRET aptasensor is composed of Mn2+-doped NaYF4:Yb3+,Er3+ UCNPs as the LRET donor, and black hole quencher 3 (BHQ3) as the acceptor. The maximum quenching efficiency is obtained by modulating the linker length, which controls the distance between the quencher and the UCNPs. Our distinctive design of LRET aptasensor allows detection of mycotoxins selectively in colored food samples within 10 min without multiple bioassay steps. We believe our single-step aptasensor has a significant potential for on-site detection of food contaminants, environmental pollutants, and biological metabolites.

Original languageEnglish
Pages (from-to)716-722
Number of pages7
JournalAnalytical Chemistry
Volume90
Issue number1
DOIs
StatePublished - 2 Jan 2018

Bibliographical note

Funding Information:
This work was financially supported by grants from the Global Research Lab (GRL) Program (NRF-2013K1A1A2A02050616) and the Midcareer Researcher Program (NRF-2017R1A2B3010816) through a National Research Foundation grant funded by the Ministry of Science ICT and Future Planning and from the Technology Innovation Program (10060155) through a grant provided by the Ministry of Trade, Industry & Energy (MI, Korea).

Funding Information:
This work was financially supported by grants from the Global Research Lab (GRL) Program (NRF-2013K1A1A2A02050616) and the Midcareer Researcher Program (NRF-2017R1A2B3010816) through a National Research Foundation grant funded by the Ministry of Science, ICT and Future Planning and from the Technology Innovation Program (10060155) through a grant provided by the Ministry of Trade, Industry & Energy (MI, Korea).

Publisher Copyright:
© 2017 American Chemical Society.

Fingerprint

Dive into the research topics of 'Single-Step LRET Aptasensor for Rapid Mycotoxin Detection'. Together they form a unique fingerprint.

Cite this