Abstract
Contamination of foods by mycotoxins is a common yet serious problem. Owing to the increase in consumption of fresh produce, consumers have become aware of food safety issues caused by mycotoxins. Therefore, rapid and sensitive mycotoxin detection is in great demand in fields such as food safety and public health. Here we report a single-step luminescence resonance energy transfer (LRET) aptasensor for mycotoxin detection. To accomplish the single-step sensor, our sensor was constructed by linking a quencher-labeled aptamer through a linker to the surface of upconversion nanoparticles (UCNPs). Our LRET aptasensor is composed of Mn2+-doped NaYF4:Yb3+,Er3+ UCNPs as the LRET donor, and black hole quencher 3 (BHQ3) as the acceptor. The maximum quenching efficiency is obtained by modulating the linker length, which controls the distance between the quencher and the UCNPs. Our distinctive design of LRET aptasensor allows detection of mycotoxins selectively in colored food samples within 10 min without multiple bioassay steps. We believe our single-step aptasensor has a significant potential for on-site detection of food contaminants, environmental pollutants, and biological metabolites.
Original language | English |
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Pages (from-to) | 716-722 |
Number of pages | 7 |
Journal | Analytical Chemistry |
Volume | 90 |
Issue number | 1 |
DOIs | |
State | Published - 2 Jan 2018 |
Bibliographical note
Funding Information:This work was financially supported by grants from the Global Research Lab (GRL) Program (NRF-2013K1A1A2A02050616) and the Midcareer Researcher Program (NRF-2017R1A2B3010816) through a National Research Foundation grant funded by the Ministry of Science ICT and Future Planning and from the Technology Innovation Program (10060155) through a grant provided by the Ministry of Trade, Industry & Energy (MI, Korea).
Funding Information:
This work was financially supported by grants from the Global Research Lab (GRL) Program (NRF-2013K1A1A2A02050616) and the Midcareer Researcher Program (NRF-2017R1A2B3010816) through a National Research Foundation grant funded by the Ministry of Science, ICT and Future Planning and from the Technology Innovation Program (10060155) through a grant provided by the Ministry of Trade, Industry & Energy (MI, Korea).
Publisher Copyright:
© 2017 American Chemical Society.