Automated data acquisition of single cell properties in an important application for labon- chip devices aimed at performing cell biology experiments. Here we present a system for both control of microenvironment variables and cell data acquisition using automated image analysis (Fig. 1). A previously presented scheme  is used to produce an alkaline environment and induce cell lysis. A custom image processing program was developed to identify single cells in the microchannel and perform morphometric measurements (sphericity, surface area, volume), as well as permeability measurements for dyes diffusing out of the cytoplasm during lysis. This system was previously applied to the study of hemolysis as a function of red blood cell shape . A similar morphometric analysis, supplemented by measurements of membrane permeability timescales, was used in order to distinguish different cell types from their alkaline lysis characteristics. Clustering by cell type is shown for five different mammalian cell types.