Single cell profiling using morphometric and permeability data: Behavior in a controlled microenvironment

Cristian Ionescu-Zanetti, Dino Di Carlo, Lee Ping Wang, Andrea Di Blas, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Automated data acquisition of single cell properties in an important application for labon- chip devices aimed at performing cell biology experiments. Here we present a system for both control of microenvironment variables and cell data acquisition using automated image analysis (Fig. 1). A previously presented scheme [1] is used to produce an alkaline environment and induce cell lysis. A custom image processing program was developed to identify single cells in the microchannel and perform morphometric measurements (sphericity, surface area, volume), as well as permeability measurements for dyes diffusing out of the cytoplasm during lysis. This system was previously applied to the study of hemolysis as a function of red blood cell shape [2]. A similar morphometric analysis, supplemented by measurements of membrane permeability timescales, was used in order to distinguish different cell types from their alkaline lysis characteristics. Clustering by cell type is shown for five different mammalian cell types.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages912-914
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 9 Oct 200513 Oct 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period9/10/0513/10/05

Keywords

  • Cell ID
  • Cell lysis
  • Cytometry
  • Hemolysis

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