Abstract
We developed a massive single-cell miRNA quantification method that analyzes the distribution of miRNA levels among thousands of single-cells at one time. We developed a novel isothermal amplification method that faithfully produces quadratic increasing signals without reaction rate decay. It can precisely quantify miRNA content with single-time-point signals instead of real-time monitoring. By using a microfluidic single-cell miRNA capturing and reaction platform, sample preparation is completed in 15 mins with 5 drops of sample or reagents by capillary flow. We quantified miRNA distributions of human breast cancer cell line MCF-7 and its doxorubicin-resistant population. The results showed miRNA sub-population changes upon drug treatment, which was not shown in ensemble averages.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 236-238 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| State | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|
Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Bibliographical note
Publisher Copyright:© 14CBMS.
Keywords
- Amplification
- Microfluidics
- MiRNA
- Single-Cell
