Single cell miRNA detection for heterogenous miRNA regulation of cancer cells

Q. Pan, X. J. Xiao, S. G. Hong, M. P. Zhao, L. P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We developed a massive single-cell miRNA quantification method that analyzes the distribution of miRNA levels among thousands of single-cells at one time. We developed a novel isothermal amplification method that faithfully produces quadratic increasing signals without reaction rate decay. It can precisely quantify miRNA content with single-time-point signals instead of real-time monitoring. By using a microfluidic single-cell miRNA capturing and reaction platform, sample preparation is completed in 15 mins with 5 drops of sample or reagents by capillary flow. We quantified miRNA distributions of human breast cancer cell line MCF-7 and its doxorubicin-resistant population. The results showed miRNA sub-population changes upon drug treatment, which was not shown in ensemble averages.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages236-238
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period26/10/1430/10/14

Keywords

  • Amplification
  • Microfluidics
  • MiRNA
  • Single-Cell

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