@inproceedings{e0be030e3b904fe3a773116bc9f7d91d,
title = "Single cell miRNA detection for heterogenous miRNA regulation of cancer cells",
abstract = "We developed a massive single-cell miRNA quantification method that analyzes the distribution of miRNA levels among thousands of single-cells at one time. We developed a novel isothermal amplification method that faithfully produces quadratic increasing signals without reaction rate decay. It can precisely quantify miRNA content with single-time-point signals instead of real-time monitoring. By using a microfluidic single-cell miRNA capturing and reaction platform, sample preparation is completed in 15 mins with 5 drops of sample or reagents by capillary flow. We quantified miRNA distributions of human breast cancer cell line MCF-7 and its doxorubicin-resistant population. The results showed miRNA sub-population changes upon drug treatment, which was not shown in ensemble averages.",
keywords = "Amplification, Microfluidics, MiRNA, Single-Cell",
author = "Q. Pan and Xiao, {X. J.} and Hong, {S. G.} and Zhao, {M. P.} and Lee, {L. P.}",
note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
year = "2014",
language = "English",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "236--238",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
}
