Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

M. J. Paik; D. T. Nguyen; Y. J. Kim; J. Y. Shin; W. Shim; E. Y. Cho; J. H. Yoon; K. R. Kim; Y. S. Lee; N. Kim; S. W. Park; G. Lee; Y. H. Ahn

doi:10.1365/s10337-010-1771-y

Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

M. J. Paik
, D. T. Nguyen
, Y. J. Kim
, J. Y. Shin
, W. Shim
, E. Y. Cho
, J. H. Yoon
, K. R. Kim
, Y. S. Lee
, N. Kim
, S. W. Park
, G. Lee
, Y. H. Ahn

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL^-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.

Original language	English
Pages (from-to)	1213-1217
Number of pages	5
Journal	Chromatographia
Volume	72
Issue number	11-12
DOIs	https://doi.org/10.1365/s10337-010-1771-y
State	Published - Dec 2010

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Communications Commission (09-basic-02) and by the National Research Foundation of Korea Grant funded by the Korean Govern ment (KRF-2008-314-E00183). This research was performed with an Agilent 6890 N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.

Keywords

Ethoxycarbonyl/pentafluoropropionyl derivatives
Gas chromatography-mass spectrometry
Melatonin, acetylserotonin and serotonin

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10.1365/s10337-010-1771-y

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@article{744e059d55a54e2d808fb6704db975de,

title = "Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine",

abstract = "Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (\% relative standard deviation = 6.9-9.4), accuracy (\% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.",

keywords = "Ethoxycarbonyl/pentafluoropropionyl derivatives, Gas chromatography-mass spectrometry, Melatonin, acetylserotonin and serotonin",

author = "Paik, \{M. J.\} and Nguyen, \{D. T.\} and Kim, \{Y. J.\} and Shin, \{J. Y.\} and W. Shim and Cho, \{E. Y.\} and Yoon, \{J. H.\} and Kim, \{K. R.\} and Lee, \{Y. S.\} and N. Kim and Park, \{S. W.\} and G. Lee and Ahn, \{Y. H.\}",

note = "Funding Information: This work was supported by a grant from the Korea Communications Commission (09-basic-02) and by the National Research Foundation of Korea Grant funded by the Korean Govern ment (KRF-2008-314-E00183). This research was performed with an Agilent 6890 N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.",

year = "2010",

month = dec,

doi = "10.1365/s10337-010-1771-y",

language = "English",

volume = "72",

pages = "1213--1217",

journal = "Chromatographia",

issn = "0009-5893",

publisher = "Springer",

number = "11-12",

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TY - JOUR

T1 - Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

AU - Paik, M. J.

AU - Nguyen, D. T.

AU - Kim, Y. J.

AU - Shin, J. Y.

AU - Shim, W.

AU - Cho, E. Y.

AU - Yoon, J. H.

AU - Kim, K. R.

AU - Lee, Y. S.

AU - Kim, N.

AU - Park, S. W.

AU - Lee, G.

AU - Ahn, Y. H.

N1 - Funding Information: This work was supported by a grant from the Korea Communications Commission (09-basic-02) and by the National Research Foundation of Korea Grant funded by the Korean Govern ment (KRF-2008-314-E00183). This research was performed with an Agilent 6890 N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.

PY - 2010/12

Y1 - 2010/12

N2 - Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.

AB - Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.

KW - Ethoxycarbonyl/pentafluoropropionyl derivatives

KW - Gas chromatography-mass spectrometry

KW - Melatonin, acetylserotonin and serotonin

UR - https://www.scopus.com/pages/publications/78651303868

U2 - 10.1365/s10337-010-1771-y

DO - 10.1365/s10337-010-1771-y

M3 - Article

AN - SCOPUS:78651303868

SN - 0009-5893

VL - 72

SP - 1213

EP - 1217

JO - Chromatographia

JF - Chromatographia

IS - 11-12

ER -

Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

Abstract

Bibliographical note

Keywords

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