Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

M. J. Paik; D. T. Nguyen; Y. J. Kim; J. Y. Shin; W. Shim; E. Y. Cho; J. H. Yoon; K. R. Kim; Y. S. Lee; N. Kim; S. W. Park; G. Lee; Y. H. Ahn

doi:10.1365/s10337-010-1771-y

Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

M. J. Paik, D. T. Nguyen, Y. J. Kim, J. Y. Shin, W. Shim, E. Y. Cho, J. H. Yoon, K. R. Kim, Y. S. Lee, N. Kim, S. W. Park, G. Lee, Y. H. Ahn

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL^-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.

Original language	English
Pages (from-to)	1213-1217
Number of pages	5
Journal	Chromatographia
Volume	72
Issue number	11-12
DOIs	https://doi.org/10.1365/s10337-010-1771-y
State	Published - Dec 2010

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Communications Commission (09-basic-02) and by the National Research Foundation of Korea Grant funded by the Korean Govern ment (KRF-2008-314-E00183). This research was performed with an Agilent 6890 N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.

Keywords

Ethoxycarbonyl/pentafluoropropionyl derivatives
Gas chromatography-mass spectrometry
Melatonin, acetylserotonin and serotonin

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10.1365/s10337-010-1771-y

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title = "Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine",

abstract = "Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.",

keywords = "Ethoxycarbonyl/pentafluoropropionyl derivatives, Gas chromatography-mass spectrometry, Melatonin, acetylserotonin and serotonin",

author = "Paik, {M. J.} and Nguyen, {D. T.} and Kim, {Y. J.} and Shin, {J. Y.} and W. Shim and Cho, {E. Y.} and Yoon, {J. H.} and Kim, {K. R.} and Lee, {Y. S.} and N. Kim and Park, {S. W.} and G. Lee and Ahn, {Y. H.}",

note = "Funding Information: This work was supported by a grant from the Korea Communications Commission (09-basic-02) and by the National Research Foundation of Korea Grant funded by the Korean Govern ment (KRF-2008-314-E00183). This research was performed with an Agilent 6890 N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.",

year = "2010",

month = dec,

doi = "10.1365/s10337-010-1771-y",

language = "English",

volume = "72",

pages = "1213--1217",

journal = "Chromatographia",

issn = "0009-5893",

publisher = "Springer Vieweg",

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T1 - Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

AU - Paik, M. J.

AU - Nguyen, D. T.

AU - Kim, Y. J.

AU - Shin, J. Y.

AU - Shim, W.

AU - Cho, E. Y.

AU - Yoon, J. H.

AU - Kim, K. R.

AU - Lee, Y. S.

AU - Kim, N.

AU - Park, S. W.

AU - Lee, G.

AU - Ahn, Y. H.

N1 - Funding Information: This work was supported by a grant from the Korea Communications Commission (09-basic-02) and by the National Research Foundation of Korea Grant funded by the Korean Govern ment (KRF-2008-314-E00183). This research was performed with an Agilent 6890 N GC interfaced to an Agilent 5975B MSD in Cooperative Center for Research Facilities of Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.

PY - 2010/12

Y1 - 2010/12

N2 - Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.

AB - Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.

KW - Ethoxycarbonyl/pentafluoropropionyl derivatives

KW - Gas chromatography-mass spectrometry

KW - Melatonin, acetylserotonin and serotonin

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AN - SCOPUS:78651303868

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JO - Chromatographia

JF - Chromatographia

IS - 11-12

ER -

Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

Abstract

Bibliographical note

Keywords

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