Simultaneous GC-MS analysis of melatonin and its precursors as ethoxycarbonyl/pentafluoropropionyl derivatives in rat urine

M. J. Paik, D. T. Nguyen, Y. J. Kim, J. Y. Shin, W. Shim, E. Y. Cho, J. H. Yoon, K. R. Kim, Y. S. Lee, N. Kim, S. W. Park, G. Lee, Y. H. Ahn

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7 Scopus citations

Abstract

Melatonin is synthesized from acetylserotonin, which is a deacetylated product of serotoinin. Simultaneous analysis of melatonin and its precursors in rat urine was performed using their (N,O)-ethoxycarbonyl/N-pentafluoropropionyl (EOC/PFP) derivatives by gas chromatography-mass spectrometry (GC-MS). The mass spectral data of acetylserotonin as mono-O-EOC/mono-N-PFP and serotonin as di-(N,O)-EOC/di-N-PFP derivatives were newly established. This method exhibited good linearity (r ≥ 0.996), repeatability (% relative standard deviation = 6.9-9.4), accuracy (% relative error = -8.9 to 8.6) with detection limits of 0.005 to 0.03 ng mL-1. This method was developed for the simultaneous measurement of melatonin and its precursors in rat urine.

Original languageEnglish
Pages (from-to)1213-1217
Number of pages5
JournalChromatographia
Volume72
Issue number11-12
DOIs
StatePublished - Dec 2010

Keywords

  • Ethoxycarbonyl/pentafluoropropionyl derivatives
  • Gas chromatography-mass spectrometry
  • Melatonin, acetylserotonin and serotonin

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