MicroRNAs (miRNAs) are small, endogenous, single-stranded, noncoding RNAs. Circulating miRNAs are being considered as promising disease biomarkers. Indeed, single miRNAs have been associated with a wide variety of disease conditions and can target multiple mRNAs; therefore, several miRNAs may be simultaneously involved in disease progression and development. In this study, we developed a capillary electrophoresis with dual laser-induced fluorescence (CE with dual LIF) method using two color laser excitations for simultaneous determination of multiple miRNAs. Target miRNAs were hybridized with 6-FAM- or Cy5-labeled DNA probes for simultaneous determination of multiple miRNAs at excitation wavelengths of 488 and 635. nm. The hybridized miRNAs were separated using CE with dual LIF and detected within 13. min at excitation wavelengths of 488 and 635. nm without any interference or crosstalk. Additionally, the proposed approach was used successfully to detect and evaluate levels of several endogenous miRNAs from lung cancer cell lines. These results showed the potential of CE with dual LIF for fast, specific, simultaneous analysis of multiple miRNAs in cell extracts, biofluids, and tissues.
Bibliographical noteFunding Information:
We gratefully acknowledge the support of the GRL Program ( 20110021713 ) from the National Research Foundation of Korea and an institutional grant from Korea Institute of Science and Technology ( 2E23810 and 2E24080 ).
- CE with dual LIF
- Clinical diagnosis
- Lung cancer
- Multiple detection