Simultaneous detection of multiple microRNAs for expression profiles of microRNAs in lung cancer cell lines by capillary electrophoresis with dual laser-induced fluorescence

Eunmi Ban, Dong Kyu Chae, Eun Joo Song

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

MicroRNAs (miRNAs) are small, endogenous, single-stranded, noncoding RNAs. Circulating miRNAs are being considered as promising disease biomarkers. Indeed, single miRNAs have been associated with a wide variety of disease conditions and can target multiple mRNAs; therefore, several miRNAs may be simultaneously involved in disease progression and development. In this study, we developed a capillary electrophoresis with dual laser-induced fluorescence (CE with dual LIF) method using two color laser excitations for simultaneous determination of multiple miRNAs. Target miRNAs were hybridized with 6-FAM- or Cy5-labeled DNA probes for simultaneous determination of multiple miRNAs at excitation wavelengths of 488 and 635. nm. The hybridized miRNAs were separated using CE with dual LIF and detected within 13. min at excitation wavelengths of 488 and 635. nm without any interference or crosstalk. Additionally, the proposed approach was used successfully to detect and evaluate levels of several endogenous miRNAs from lung cancer cell lines. These results showed the potential of CE with dual LIF for fast, specific, simultaneous analysis of multiple miRNAs in cell extracts, biofluids, and tissues.

Original languageEnglish
Pages (from-to)195-199
Number of pages5
JournalJournal of Chromatography A
Volume1315
DOIs
StatePublished - 8 Nov 2013

Bibliographical note

Funding Information:
We gratefully acknowledge the support of the GRL Program ( 20110021713 ) from the National Research Foundation of Korea and an institutional grant from Korea Institute of Science and Technology ( 2E23810 and 2E24080 ).

Keywords

  • Biomarker
  • CE with dual LIF
  • Clinical diagnosis
  • Lung cancer
  • MiRNA
  • Multiple detection

Fingerprint

Dive into the research topics of 'Simultaneous detection of multiple microRNAs for expression profiles of microRNAs in lung cancer cell lines by capillary electrophoresis with dual laser-induced fluorescence'. Together they form a unique fingerprint.

Cite this