Abstract
An efficient method for the simultaneous analysis of seven 2-hydroxy fatty acids (2-HFAs) as tert-butyldimethylsilyl (TBDMS) derivative was developed by gas chromatography-mass spectrometry in selected ion monitoring mode. New mass spectral data on 2-hydroxycapric, 2-hydroxypalmitic, 2-hydroxystearic and 2-hydroxybehenic acids as di-TBDMS derivatives for hydroxyl and carboxyl groups were built. Under the optimal conditions, the present method showed a good correlation coefficient (r≥0.999) in the range of 0.01-0.5μg. The precision showed low relative standard deviation of <10%, and the accuracy (percentage relative error) varied from -5.2 to 0.3 for the seven 2-HFAs studied. Recovery rates of all 2-HFAs were ≥93.2% with good precision. When applied to normal human plasma, seven 2-HFAs were positively identified. Therefore, the present efficient method will be useful for simultaneous analysis of 2-HFAs in plasma.
Original language | English |
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Pages (from-to) | 156-160 |
Number of pages | 5 |
Journal | Biomedical Chromatography |
Volume | 29 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jan 2015 |
Keywords
- 2-hydroxy fatty acid
- Gas chromatography-mass spectrometry
- Plasma
- tert-butyldimethylsilylation