Silver nanoparticles enhance thrombus formation through increased platelet aggregation and procoagulant activity

Eun Ah Jun, Kyung Min Lim, Keunyoung Kim, Ok Nam Bae, Ji Yoon Noh, Kyu Hyuck Chung, Jin Ho Chung

Research output: Contribution to journalArticlepeer-review

119 Scopus citations


Despite the wide use of silver nanoparticles (nano Ag), its toxicity still remains poorly understood. In this report, nano Ag induced an increase in platelet aggregation and procoagulant activation which are the key contributors to thrombotic diseases. In freshly isolated human platelets, nano Ag induced platelet aggregation and procoagulant activation evident by increased phosphatidylserine exposure and thrombin generation. Interestingly, the sub-threshold level of thrombin enhanced nano Ag-induced platelet activation significantly indicating that the prothrombotic effects of nano Ag might be further potentiated in activated platelets. An increase in intracellular calcium mediated nano Ag induced platelet activation and P-selectin expression, and serotonin release was also enhanced by nano Ag. Consistent with the in vitro results, exposure to nano Ag (0.05-0.1 mg/kg i.v. or 5-10 mg/kg intratracheal instillation) in vivo enhanced venous thrombus formation, platelet aggregation, and phosphatidylserine externalization ex vivo in rats suggesting that nano Ag, indeed, does enhance thrombus formation through platelet activation.

Original languageEnglish
Pages (from-to)157-167
Number of pages11
Issue number2
StatePublished - Jun 2011

Bibliographical note

Funding Information:
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (20090076807).


  • Silver nanoparticles
  • cardiovascular toxicity
  • platelet aggregation
  • platelet procoagulant activity
  • thrombus formation


Dive into the research topics of 'Silver nanoparticles enhance thrombus formation through increased platelet aggregation and procoagulant activity'. Together they form a unique fingerprint.

Cite this