The effect of silkworm hemolymph on baculovirus-induced insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity; however, its addition after the infection was less effective. This can be explained by the higher transfer rate of silkworm hemolymph which is caused by endocytosis during the virus internalization step. The delayed cell death due to silkworm hemolymph was not caused by an inhibition of the virus attachment and internalization steps. The apoptosis was analyzed using DNA fragmentation and TUNEL assays, and the resulting data confirm that silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis. (C) 2000 Academic Press.
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 29 Apr 2000|
Bibliographical noteFunding Information:
The authors acknowledge the financial support of the Korea Science and Engineering Foundation (981-1105-024-2). This work was supported in part by the Brain Korea 21 Program from the Ministry of Education. Silkworms were kindly provided by Dr. Sam-Eun Kim, Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Suwon, Korea.