Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells

Won Jong Rhee, Eun Jeong Kim, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by baculovirus but also by chemical inducers. This indicates that silkworm hemolymph contains anti-apoptotic components that work directly in insect cell apoptosis without any booster expression of baculoviral genes. With the analysis of Sf-caspase-1 activity, it was found that the inhibitory effect of silkworm hemolymph works in a further upstream step than the Sf-caspase-1 activation step.

Original languageEnglish
Pages (from-to)779-783
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume295
Issue number4
DOIs
StatePublished - 2002

Bibliographical note

Funding Information:
The authors acknowledge the financial support of the Korea Science and Engineering Foundation through the Nano Bio-Electronic and System Center, Seoul National University, Seoul, Korea. Silkworms were provided by Dr. Sam-Eun Kim, Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Suwon, Korea.

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