@inproceedings{60c4bfe1194e4134b448991e9c351354,
title = "Signaling and polarized response of budding yeast to gradient of mating pheromone",
abstract = "The yeast cell is known to sense a minute difference in pheromone concentration but the intracellular pathway is not fully understood yet. We utilized microfludic device that generates concentration gradient as single cell imaging platform to evaluate proteins related to the mechanisms. Pressure-driven flow of high and low pheromone concentration achieved stable gradient, and the micro barrier nearby culture chamber reduced the fluidic perturbation on cell orientation. The orientation angle and morphological change were quantified under the gradient. This microfluidic assay showed phenotypic diversity and demonstrated the lack of sensitivity of the Far1p mutant compared to the wild type cells.",
keywords = "Gradient, Mating projection, Quantitative Single Cell Analysis",
author = "Lee, {Sung S.} and Serge Pelet and Lee, {Luke P.} and Matthias Peter",
year = "2009",
language = "English",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "636--638",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
}