SIFamide and SIFamide receptor define a novel neuropeptide signaling to promote sleep in Drosophila

Sangjin Park, Jun Young Sonn, Yangkyun Oh, Chunghun Lim, Joonho Choe

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


SIFamide receptor (SIFR) is a Drosophila G protein-coupled receptor for the neuropeptide SIFamide (SIFa). Although the sequence and spatial expression of SIFa are evolutionarily conserved among insect species, the physiological function of SIFa/SIFR signaling remains elusive. Here, we provide genetic evidence that SIFa and SIFR promote sleep in Drosophila. Either genetic ablation of SIFa-expressing neurons in the pars intercerebralis (PI) or pan-neuronal depletion of SIFa expression shortened baseline sleep and reduced sleep-bout length, suggesting that it caused sleep fragmentation. Consistently, RNA interference-mediated knockdown of SIFR expression caused short sleep phenotypes as observed in SIFa-ablated or depleted flies. Using a panel of neuron-specific Gal4 drivers, we further mapped SIFR effects to subsets of PI neurons. Taken together, these results reveal a novel physiological role of the neuropeptide SIFa/SIFR pathway to regulate sleep through sleep-promoting neural circuits in the PI of adult fly brains.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalMolecules and Cells
Issue number4
StatePublished - Apr 2014


  • Drosophila melanogaster
  • Pars intercerebralis
  • SIFamide
  • SIFamide receptor
  • Sleep


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