Shrimp miR-965 transfers tumoricidal mitochondria

Hyueyun Kim, Ji Ha Choi, Chang Mo Moon, Jihee Lee, Minna Woo, Minsuk Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Micro RNA of Marsupenaeus japonicas has been known to promote apoptosis of tumor cells. However, the detailed mechanisms are not well understood. Results: Using tomographic microscope, which can detect the internal structure of cells, we observed breast tumor cells following treatment of the miRNA. Intriguingly, we found that mitochondria migrate to an adjacent tumor cells through a tunneling nanotube. To recapitulate this process, we engineered a microfluidic device through which mitochondria were transferred. We show that this mitochondrial transfer process released endonuclease G (Endo G) into tumor cells, which we referred to herein as unsealed mitochondria. Importantly, Endo G depleted mitochondria alone did not have tumoricidal effects. Moreover, unsealed mitochondria had synergistic apoptotic effects with subtoxic dose of doxorubicin thereby mitigating cardiotoxicity. Conclusions: Together, we show that the mitochondrial transfer through microfluidics can provide potential novel strategies towards tumor cell death.

Original languageEnglish
Article number16
JournalBiological Procedures Online
Volume24
Issue number1
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant (2020R1A5A2019210 and 2019R1C1C1003384) funded by the Korean government (MSIT).

Publisher Copyright:
© 2022, The Author(s).

Keywords

  • Breast tumor
  • Cotton candy
  • Microfluidics
  • Mitochondria
  • Refractive index
  • Tomographic microscope
  • Tunneling nanotube

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