Short-term hyperhomocysteinemia-induced oxidative stress activates retinal glial cells and increases vascular endothelial growth factor expression in rat retina

Insun Lee, Hwayoung Lee, Ji Myung Kim, Hye Chae Eun, Jung Kim Soo, Namsoo Chang

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Hyperhomocysteinemia is associated with an increase in the incidence of vascular diseases, including retinal vascular diseases. We examined the effects of high plasma levels of homocysteine on retinal glial cells and vascular endothelial growth factor (VEGF) expression. Male Sprague-Dawley rats were fed either a 3.0 g/kg homocystine diet or a control diet for 2 week. The homocystine-diet group had higher plasma levels of homocysteine and thiobarbituric acid reactive substances (TBARSs) and lower plasma levels of folate, retinol, α-tocopherol, and retinal expression of CuZn superoxide dismutase (SOD) than the controls. The rats fed the homocystine-diet showed an increase in vimentin, glial fibrillary acidic protein (GFAP), and VEGF immunoreactivity in the retina as compared to the controls. The increase in vimentin immunoreactivity in the hyperhomocysteinemic rats was correlated with changes in GFAP immunoreactivity in astrocytes within the ganglion cell layer. We found for the first time that short-term hyperhomocysteinemia-induced oxidative stress activates retinal glial cells and increases VEGF expression in the retina.

Original languageEnglish
Pages (from-to)1203-1210
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number5
DOIs
StatePublished - 2007

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Research Grant Foundation funded by the Korean Government (KRF-2005-C00088-I00261).

Keywords

  • Glial fibrillary acidic protein
  • Hyperhomocysteinemia
  • Retina
  • Vascular endothelial growth factor
  • Vimentin

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