@article{182bf1e2322543df924cc943c435ff11,
title = "Serine protease in a bred variety of oriental melon (Cucumis melo L. var. makuwa) curtails vascular thrombosis by balancing hemostasis and fibrinolysis in a rodent model",
abstract = "Maintaining proper blood flow is critical in promoting good health. This study examined whether oral consumption of a bred variety of oriental melon for high serine protease (OMS) dampened collagen/epinephrine (CE)-induced vascular thrombosis in rats in comparison with a regular cultivar (OM). Freeze-dried OM or OMS were administered by oral gavage for 4 weeks. In a CE-induced thrombotic condition, OMS significantly reduced thrombogenesis by inhibiting both cyclooxygenase-1-mediated thromboxane B2 production and intrinsic blood coagulation pathways. It also stimulated fibrin clot lysis by activating tissue-type plasminogen activator. Besides, OMS restored CE-mediated alterations in adhesion molecules and endothelial nitric oxide synthase, thereby resulting in vasodilation. OM showed similar tendencies, but not statistically equivalent. The current findings, for the first time, suggest that plant SP from OMS may have the potential to protect endothelial and vascular dysfunctions that can be encountered by unintentional exposure to thrombotic risk factors in daily life.",
keywords = "Anti-thrombotic, Cucumis melo L. var. makuwa, Oriental melon, Pro-fibrinolytic, Serine protease",
author = "Hendryanti, {Dea N.} and Hyeyun Jeong and Kim, {Ji Yeon} and Oran Kwon",
note = "Funding Information: We thank Nongwoo Bio Co. Ltd (Gyunggi-do, Republic of Korea) for the insight and generous support with the test materials; We also thank Professor Deung-Chan Lee (Kangwon National University, Kangwondaehakgil 1, Chuncheon, Republic of Korea) for the generous provision of the analytical data on the ELISA using a polyclonal antibody against SP. The authors declare no competing financial interest. The study was approved by the Institutional Animal Care and Use Committees of Ewha Womans University, Seoul, Korea (Approval number: 18-013) and conducted in compliance with the National Research Council Guidelines. This work was supported by the Agri-bio Industry Technology Development Program of the Ministry of Agriculture, Food, and Rural Affairs through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry [grant number 112132-05]; and the Bio-Synergy Research Project of the Ministry of Science and ICT through the National Research Foundation [grant number NRF-2012M3A9C4048761]. Funding Information: This work was supported by the Agri-bio Industry Technology Development Program of the Ministry of Agriculture, Food, and Rural Affairs through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry [grant number 112132-05 ]; and the Bio-Synergy Research Project of the Ministry of Science and ICT through the National Research Foundation [grant number NRF-2012M3A9C4048761 ]. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = may,
doi = "10.1016/j.jff.2020.103925",
language = "English",
volume = "68",
journal = "Journal of Functional Foods",
issn = "1756-4646",
publisher = "Elsevier Ltd.",
}