Separation of two major chalcones from Angelica keiskei by high-speed counter-current chromatography

Yun Seo Kil, Joo Won Nam, Jun Lee, Eun Kyoung Seo

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Abstract Angelica keiskei (Shin-sun cho) is an edible higher plant with the beneficial preventive effects on cancer, hypertension, and coronary heart disease. Two bioactive chalcones of Shin-sun cho, xanthoangelol (1) and 4-hydroxyderricin (2), were separated simultaneously by using high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-EtOAc-MeOH-H2O (9:5:9:4). Only nonconsuming processes, solvent fractionations and Sephadex LH-20 column chromatography, were conducted as presteps. Xanthoangelol (1, 35.9 mg, 99.9 % purity at 254 and 365 nm) and 4-hydroxyderricin (2, 4.4 mg, 98.7 % purity at 254 nm and 98.8 % purity at 365 nm) were successfully purified from 70 mg of the processed extract from A. keiskei. The structures of two compounds were confirmed by 1H- and 13C-NMR analysis.

Original languageEnglish
Article number530
Pages (from-to)1506-1511
Number of pages6
JournalArchives of Pharmacal Research
Volume38
Issue number8
DOIs
StatePublished - 8 Aug 2015

Keywords

  • 4-Hydroxyderricin
  • Angelica keiskei
  • Chalcone
  • HSCCC
  • Umbelliferae
  • Xanthoangelol

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