TY - JOUR
T1 - Separation of calcareous corpuscles from plerocercoids of Spirometra mansoni and their binding proteins
AU - Yang, H. J.
N1 - Funding Information:
Acknowledgement This research was supported by a grant from the Cheju National University Medical Research Fund.
PY - 2000
Y1 - 2000
N2 - Calcareous corpuscles, known as mineral concretions in Platyhelminthes, may have special biological functions. In the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of Spirometra mansoni (sparganum) using Ficoll. Purified calcareous corpuscles were reacted with crude extracts of Platyhelminthes, including sparganum. Proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kDa. A 95-kDa molecule was found to bind specifically in cystic fluid of Cysticercus cellulosae, as was a 40-kDa protein in crude extracts of Taenia saginata and a 27-kDa molecule in crude extracts of Paragonimus westermani and Clonorchis sinensis, respectively. This finding suggests that calcareous corpuscles might have a binding activity to proteins in crude extracts of sparganum.
AB - Calcareous corpuscles, known as mineral concretions in Platyhelminthes, may have special biological functions. In the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of Spirometra mansoni (sparganum) using Ficoll. Purified calcareous corpuscles were reacted with crude extracts of Platyhelminthes, including sparganum. Proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kDa. A 95-kDa molecule was found to bind specifically in cystic fluid of Cysticercus cellulosae, as was a 40-kDa protein in crude extracts of Taenia saginata and a 27-kDa molecule in crude extracts of Paragonimus westermani and Clonorchis sinensis, respectively. This finding suggests that calcareous corpuscles might have a binding activity to proteins in crude extracts of sparganum.
UR - http://www.scopus.com/inward/record.url?scp=0033830148&partnerID=8YFLogxK
U2 - 10.1007/s004360000241
DO - 10.1007/s004360000241
M3 - Article
C2 - 11002990
AN - SCOPUS:0033830148
SN - 0932-0113
VL - 86
SP - 781
EP - 782
JO - Parasitology Research
JF - Parasitology Research
IS - 9
ER -