Separation of calcareous corpuscles from plerocercoids of Spirometra mansoni and their binding proteins

H. J. Yang

doi:10.1007/s004360000241

Separation of calcareous corpuscles from plerocercoids of Spirometra mansoni and their binding proteins

H. J. Yang

Department of Parasitology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Calcareous corpuscles, known as mineral concretions in Platyhelminthes, may have special biological functions. In the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of Spirometra mansoni (sparganum) using Ficoll. Purified calcareous corpuscles were reacted with crude extracts of Platyhelminthes, including sparganum. Proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kDa. A 95-kDa molecule was found to bind specifically in cystic fluid of Cysticercus cellulosae, as was a 40-kDa protein in crude extracts of Taenia saginata and a 27-kDa molecule in crude extracts of Paragonimus westermani and Clonorchis sinensis, respectively. This finding suggests that calcareous corpuscles might have a binding activity to proteins in crude extracts of sparganum.

Original language	English
Pages (from-to)	781-782
Number of pages	2
Journal	Parasitology Research
Volume	86
Issue number	9
DOIs	https://doi.org/10.1007/s004360000241
State	Published - 2000

Bibliographical note

Funding Information:
Acknowledgement This research was supported by a grant from the Cheju National University Medical Research Fund.

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title = "Separation of calcareous corpuscles from plerocercoids of Spirometra mansoni and their binding proteins",

abstract = "Calcareous corpuscles, known as mineral concretions in Platyhelminthes, may have special biological functions. In the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of Spirometra mansoni (sparganum) using Ficoll. Purified calcareous corpuscles were reacted with crude extracts of Platyhelminthes, including sparganum. Proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kDa. A 95-kDa molecule was found to bind specifically in cystic fluid of Cysticercus cellulosae, as was a 40-kDa protein in crude extracts of Taenia saginata and a 27-kDa molecule in crude extracts of Paragonimus westermani and Clonorchis sinensis, respectively. This finding suggests that calcareous corpuscles might have a binding activity to proteins in crude extracts of sparganum.",

author = "Yang, {H. J.}",

note = "Funding Information: Acknowledgement This research was supported by a grant from the Cheju National University Medical Research Fund.",

year = "2000",

doi = "10.1007/s004360000241",

language = "English",

volume = "86",

pages = "781--782",

journal = "Parasitology Research",

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T1 - Separation of calcareous corpuscles from plerocercoids of Spirometra mansoni and their binding proteins

AU - Yang, H. J.

N1 - Funding Information: Acknowledgement This research was supported by a grant from the Cheju National University Medical Research Fund.

PY - 2000

Y1 - 2000

N2 - Calcareous corpuscles, known as mineral concretions in Platyhelminthes, may have special biological functions. In the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of Spirometra mansoni (sparganum) using Ficoll. Purified calcareous corpuscles were reacted with crude extracts of Platyhelminthes, including sparganum. Proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kDa. A 95-kDa molecule was found to bind specifically in cystic fluid of Cysticercus cellulosae, as was a 40-kDa protein in crude extracts of Taenia saginata and a 27-kDa molecule in crude extracts of Paragonimus westermani and Clonorchis sinensis, respectively. This finding suggests that calcareous corpuscles might have a binding activity to proteins in crude extracts of sparganum.

AB - Calcareous corpuscles, known as mineral concretions in Platyhelminthes, may have special biological functions. In the present study we succeeded in the quantitative separation of calcareous corpuscles in plerocercoids of Spirometra mansoni (sparganum) using Ficoll. Purified calcareous corpuscles were reacted with crude extracts of Platyhelminthes, including sparganum. Proteins commonly binding to calcareous corpuscles were molecules of 10, 17, 22, and 35 kDa. A 95-kDa molecule was found to bind specifically in cystic fluid of Cysticercus cellulosae, as was a 40-kDa protein in crude extracts of Taenia saginata and a 27-kDa molecule in crude extracts of Paragonimus westermani and Clonorchis sinensis, respectively. This finding suggests that calcareous corpuscles might have a binding activity to proteins in crude extracts of sparganum.

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Separation of calcareous corpuscles from plerocercoids of Spirometra mansoni and their binding proteins

Abstract

Bibliographical note

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