Sensors for In Situ Real-Time Fluorescence Imaging of Enzymes

Xiaofeng Wu; Haidong Li; Eunhye Lee; Juyoung Yoon

doi:10.1016/j.chempr.2020.10.010

Sensors for In Situ Real-Time Fluorescence Imaging of Enzymes

Xiaofeng Wu, Haidong Li, Eunhye Lee, Juyoung Yoon

Chemistry & Nanoscience

Research output: Contribution to journal › Review article › peer-review

34 Scopus citations

Abstract

Challenges and opportunities: • In situ real-time fluorescence imaging is an extremely useful method for researchers to observe the dynamics of enzymes and their interactions with other species in the biosystem • Developing new methods with high binding efficiency toward enzyme for in situ real-time imaging enzyme remains a great challenge • Enzyme- and photoactivatable fluorescent sensors bearing a diazoindanone moiety may prove to be a promising tool for in situ real-time imaging with high resolution of enzymes

Original language	English
Pages (from-to)	2893-2901
Number of pages	9
Journal	Chem
Volume	6
Issue number	11
DOIs	https://doi.org/10.1016/j.chempr.2020.10.010
State	Published - 5 Nov 2020

Keywords

SDG3: Good health and well-being
dynamics
enzymes
fluorescence imaging
in situ
real-time

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title = "Sensors for In Situ Real-Time Fluorescence Imaging of Enzymes",

abstract = "Challenges and opportunities: • In situ real-time fluorescence imaging is an extremely useful method for researchers to observe the dynamics of enzymes and their interactions with other species in the biosystem • Developing new methods with high binding efficiency toward enzyme for in situ real-time imaging enzyme remains a great challenge • Enzyme- and photoactivatable fluorescent sensors bearing a diazoindanone moiety may prove to be a promising tool for in situ real-time imaging with high resolution of enzymes",

keywords = "SDG3: Good health and well-being, dynamics, enzymes, fluorescence imaging, in situ, real-time",

author = "Xiaofeng Wu and Haidong Li and Eunhye Lee and Juyoung Yoon",

note = "Funding Information: We are grateful to the editor for his kind invitation to submit this perspective. This study was supported financially by a National Research Foundation of Korea (NRF) grant funded by the Korean Government ( MSIP ) (no. 2 012R1A3A2048814 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

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doi = "10.1016/j.chempr.2020.10.010",

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T1 - Sensors for In Situ Real-Time Fluorescence Imaging of Enzymes

AU - Wu, Xiaofeng

AU - Li, Haidong

AU - Lee, Eunhye

AU - Yoon, Juyoung

N1 - Funding Information: We are grateful to the editor for his kind invitation to submit this perspective. This study was supported financially by a National Research Foundation of Korea (NRF) grant funded by the Korean Government ( MSIP ) (no. 2 012R1A3A2048814 ). Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/11/5

Y1 - 2020/11/5

N2 - Challenges and opportunities: • In situ real-time fluorescence imaging is an extremely useful method for researchers to observe the dynamics of enzymes and their interactions with other species in the biosystem • Developing new methods with high binding efficiency toward enzyme for in situ real-time imaging enzyme remains a great challenge • Enzyme- and photoactivatable fluorescent sensors bearing a diazoindanone moiety may prove to be a promising tool for in situ real-time imaging with high resolution of enzymes

AB - Challenges and opportunities: • In situ real-time fluorescence imaging is an extremely useful method for researchers to observe the dynamics of enzymes and their interactions with other species in the biosystem • Developing new methods with high binding efficiency toward enzyme for in situ real-time imaging enzyme remains a great challenge • Enzyme- and photoactivatable fluorescent sensors bearing a diazoindanone moiety may prove to be a promising tool for in situ real-time imaging with high resolution of enzymes

KW - SDG3: Good health and well-being

KW - dynamics

KW - enzymes

KW - fluorescence imaging

KW - in situ

KW - real-time

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DO - 10.1016/j.chempr.2020.10.010

M3 - Review article

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SN - 2451-9308

VL - 6

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EP - 2901

JO - Chem

JF - Chem

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ER -

Sensors for In Situ Real-Time Fluorescence Imaging of Enzymes

Abstract

Keywords

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