@inproceedings{de366f6c304245fab49eac3120b6903c,
title = "Self-raised circular orifices for lateral patch-clamping array chips",
abstract = "We have developed a novel technique to build three-dimensional (3-D) self-raised lateral microfluidic cell trapping junctions in polydimethylsiloxane (PDMS) microfluidic networks for patch clamp experiments. As far as cell deformation is concerned, a 3-D cell trapping junction that has symmetrical space around a patching orifice is desirable since it induces symmetrical deformation of the cell and reduced gap between the cellular membrane and patching orifice. The fabricated 3-D junction is relatively raised from the edge of the channel. Evidence of the hydrodynamic placement of mammalian cells at the junctions and measurements of patch sealing resistance is presented.",
keywords = "Cells, Ion channels, Microfluidics, Patch-clamp",
author = "Jeonggi Seo and Lee, {Luke P.}",
year = "2005",
language = "English",
isbn = "0974361119",
series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Transducer Research Foundation",
pages = "1215--1217",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",
note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",
}